"22"^^ . "7118"^^ . . . "3885"^^ . . . . "Using DNA-microarray technology, the transcirptome maps will be determined for several normal and tumor cell populations in different stages of progression. Comparison ot maps should allow us to find out combinations of genes related to cancerogenesis. The structure of chromosomes will be inbestigated in interphase 3D-fixed nuclei by means of FISH and high-resolution cytometry. Changes of the structure re latively to the normal tissue xill be compared with changes of gene expression. Conclusion will be made on the relationship between the structure of chromosomes and expression of genes during cancerogenesis. It may give us new and relatively cheap method for the detection of oncogenic transformation in the specific tissue. The project will bring DNA-microarrays to the second laboratory in the Czech Republic, which will be important for the regional development of progressive tehnologies."@en . "3"^^ . . . "Epigeneticky kontrolovan\u00E9 zm\u011Bny exprese gen\u016F u n\u00E1dorov\u00FDch onemocn\u011Bn\u00ED"@cs . . "0"^^ . . "Pomoc\u00ED technologie DNA-mikro\u010Dip\u016F budou stanoveny transkrip\u010Dn\u00ED mapy u n\u011Bkolika populac\u00ED zdrav\u00FDch a n\u00E1dorov\u00FDch bun\u011Bk s r\u016Fzn\u00FDm stupn\u011Bm progrese. Porovn\u00E1n\u00EDm transkrip\u010Dn\u00EDch map se budeme sna\u017Eit o nalezen\u00ED kombinac\u00ED gen\u016F, pomi\u0148uj\u00EDc\u00EDch kancerogenezi. D\u00E1le bude opu\u017Eita FISH u 3D fixovan\u00FDch bun\u011Bk a cytometrie s vysok\u00FDm rozli\u0161en\u00EDm ke zji\u0161t\u011Bn\u00ED struktury chromosom\u016F v interf\u00E1zn\u00EDm j\u00E1d\u0159e. Zm\u011Bny ve struktu\u0159e oproti norm\u00E1ln\u00ED tk\u00E1ni budou porovn\u00E1ny se zm\u011Bnami exprese. Bude u\u010Din\u011Bn z\u00E1v\u011Br o m\u00ED\u0159e souvislosti mezi strukturou a expres\u00ED v pr\u016Fb\u011Bhu karcinogeneze. T\u00EDm m\u016F\u017Eeme z\u00EDskat mo\u017Enost pou\u017Eit\u00ED nov\u00E9, levn\u00E9 metody k detekci onkogenn\u00ED transformace specifick\u00E9 tk\u00E1n\u011B. Projekt p\u0159inese tak\u00E9 zaveden\u00ED techniky DNA-mikro\u010D\u00EDp\u016F na druh\u00E9m pracovi\u0161ti v \u010CR, co\u017E bude m\u00EDt v\u00FDznam pro region\u00E1ln\u00ED rozvoj progresivn\u00EDch technologi\u00ED."@cs . . "DNA-microarrays;cancer;chromosome structure;epigenetic regulation;cell differentiation"@en . . "1"^^ . "Epigenetic control of gene expression in malignant diseases"@en . "22"^^ .