"In-vitro study of molecular-genetic cuase of cell resistance against selected drugs"@en . "2008-12-31+01:00"^^ . "In-vitro studie molekul\u00E1rn\u011B-genetick\u00FDch p\u0159\u00ED\u010Din bun\u011B\u010Dn\u00E9 rezistence v\u016F\u010Di vybran\u00FDm l\u00E9\u010Div\u016Fm"@cs . . "0"^^ . . . . . "7196"^^ . "1"^^ . "2"^^ . . "1"^^ . "6751"^^ . "2005-08-01+01:00"^^ . . . . "The aim of this study is the search for and validation of more markers of drug resistance (among the known as MDR1, MRP1 or BCRP) and possible genetic predisposing factors. By comparing the gene expression profile of both the parental and resistant cell-line, the specific role of ABC transporters in emerging drug resistance in context of other genes expression will be determined. Cell-lines genetically modified to over-express selected ABC transporter will be used in functional study and the specific substrate transport will be determined. Expected result of this study is finding and verification of genes concerning multidrug resistance which should be used as a markers for drug resistance early diagnosis."@en . "2"^^ . "\u00DAkolem t\u00E9to studie je vyhled\u00E1n\u00ED a ov\u011B\u0159en\u00ED dal\u0161\u00EDch marker\u016F vzniku l\u00E9kov\u00E9 rezistence (krom\u011B ji\u017E zn\u00E1m\u00FDch, jako MDR1 nebo BCRP) a vybran\u00FDch p\u0159\u00EDpadn\u00FDch genetick\u00FDch predisponuj\u00EDc\u00EDch faktor\u016F. Z\u00EDsk\u00E1n\u00EDm rezistentn\u00ED bun\u011B\u010Dn\u00E9 linie a srovn\u00E1n\u00EDm exprese s mate\u0159skou lini\u00ED budou z\u00EDsk\u00E1na data, jejich\u017E anal\u00FDzou bude mo\u017En\u00E9 zjistit \u00FAlohu ABC transport\u00E9r\u016F p\u0159i vzniku rezistence v souvislosti se zm\u011Bnou exprese dal\u0161\u00EDch gen\u016F. P\u0159ipraven\u00E9 nep\u0159\u00EDbuzn\u00E9 bun\u011B\u010Dn\u00E9 linie transfekovan\u00E9 a zv\u00FD\u0161en\u011B exprimuj\u00EDc\u00ED dan\u00FD ABC transport\u00E9r budou pou\u017Eity p\u0159i funk\u010Dn\u00ED studii a bude ov\u011B\u0159ena schopnost dan\u00E9ho proteinu transportovat p\u0159\u00EDslu\u0161n\u00E9 l\u00E9\u010Divo. O\u010Dek\u00E1van\u00FDm v\u00FDsledkem studie je vyhled\u00E1n\u00ED a funk\u010Dn\u00ED ov\u011B\u0159en\u00ED gen\u016F pod\u00EDlej\u00EDc\u00EDch se v\u00FDraznou m\u011Brou na vzniku rezistence a pou\u017Eiteln\u00FDch d\u00E1le jako marker\u016F pro v\u010Dasnou diagnostiku vznikaj\u00EDc\u00ED l\u00E9kov\u00E9 rezistence."@cs . . . "molecular; genetics; resistance; drug; cell-line; ABC-transporter; transport"@en . .