. . "1"^^ . "DNA microarrays; histone modifications; inhibitors of deacetylases; chromatin structure; leukemia; histone deacetylases; epigenetics; Gleevec"@en . "2"^^ . . . . . "3885"^^ . . . "19"^^ . . "4022"^^ . . "Nov\u00E9 mo\u017Enosti diagnostiky leuk\u00E9mi\u00ED s vyu\u017Eit\u00EDm technologie DNA-mikro\u010Dip\u016F"@cs . "19"^^ . "0"^^ . "Bude studov\u00E1na exprese pomoc\u00ED DNA microarrays u leuk\u00E9mi\u00ED, vhodn\u00FDch kontroln\u00EDch bun\u011Bk a pro in vitro populace. Zm\u011Bny budou korelov\u00E1ny s epigenetick\u00FDmi charakteristikami chromatinu na \u00FArovni jeho modifikac\u00ED (metylace, acetylace histon\u016F, metylace DNA). Struktur\u00E1ln\u00ED charakteristiky chromatinu budou zkoum\u00E1ny v m\u00EDstech se zm\u011Bnami exprese pomoc\u00ED fluorescen\u010Dn\u00ED in situ hybridizace, spektr\u00E1ln\u00ED mikroskopie a imunoFISH. Budou testov\u00E1ny protin\u00E1dorov\u00E9 l\u00E1tky s \u00FAzk\u00FDm spektrem \u00FA\u010Dinku na \u00FArovni exprese. Vyu\u017Eit\u00EDm pokro\u010Dil\u00FDch metod vizualizace jadern\u00FDch stuktur a modern\u00ED konfok\u00E1ln\u00ED mikroskopie bude studov\u00E1n mechanismus vzniku a progrese leuk\u00E9mi\u00ED. C\u00EDlem bude navrhnout vhodn\u00E9 markry pro diagnostiku. Domn\u00EDv\u00E1me se, \u017Ee studium gen\u016F m\u011Bn\u00EDc\u00EDch expresi a epigenetick\u00FDch mechanism\u016F stoj\u00EDc\u00EDch za t\u011Bmito zm\u011Bnami n\u00E1m umo\u017En\u00ED navrhnout c\u00EDlov\u00E9 molekuly pro l\u00E9\u010Dbu."@cs . "New possibilities of diagnostics of leukemia using DNA-microarrays"@en . "Using DNA microarrays, gene expression will be studied in leukemia, in control cell populations and in cell cultures. Changes will be correlated to epigenetic characteristics of chromatin at the level of its modifications (methylation, acetylation). Structural characteristics of chromatin at sites with altered expression will be studied using fluorescence in situ hybridization (FISH), spectral microscopy and immunoFISH. Drugs with narrow spectrum of genes with altered expression wil be studied. Using advanced techniques of gene or protein visualization and top level confocal microscopy, the mechanisms of the induction and progression of leukemia will be investigated with the goal to suggest appropriate markers for diagnostics. We hope that our study of gene expression in parallel to epigenetics will allow us to propose target molecules of effective therapy."@en .