"2012-03-01+01:00"^^ . . . . . . "2275"^^ . "To clarify the etiology of diseases of important cultivated crops as well as wild growing plants associated with symptoms typical for phytoplasmas in the Czech Republic.Molecular hybridization approach for universal detection on microarray platform will be developed based on rRNA (and other) genes.Study on the presence and variability of plasmids in phytoplasmas inducing different level of disease symptoms."@en . "0"^^ . "2013-05-31+01:00"^^ . "Anal\u00FDza genomov\u00FDch komponent fytoplazem a v\u00FDvoj microarrays pro jejich detekci a management"@cs . "0"^^ . "750"^^ . "0"^^ . "Analysis of phytoplasma genomic components and development of a microarray for their determination and management."@en . . "0"^^ . . . "1"^^ . . "Phytoplasma; plasmids; molecular detection; microarrays"@en . "C\u00EDlem projektu je identifikovat izol\u00E1ty fytoplazem z kulturn\u00EDch a plan\u011B rostouc\u00EDch rostlin, bylin i d\u0159evin. D\u00E1le vytvo\u0159it p\u0159enosem z p\u016Fvodn\u00EDch symptomatick\u00FDch rostlin roubov\u00E1n\u00EDm, nebo pomoc\u00ED parazitick\u00E9 rostliny Cuscuta sp. na indik\u00E1torov\u00E9 rostliny Catharanthus roseus, sb\u00EDrku fytoplazem v tk\u00E1\u0148ov\u00FDch kultur\u00E1ch, kter\u00E1 umo\u017En\u00ED trvalou dostupnost fytoplazem pro jejich studium na molekul\u00E1rn\u00ED \u00FArovni. Prok\u00E1zat molekul\u00E1rn\u00EDmi technikami obecnou p\u0159\u00EDtomnost plazmid\u016F v r\u016Fzn\u00FDch druz\u00EDch fytoplazem, zjistit jejich variabilitu v konkr\u00E9tn\u00EDm genu. Z\u00EDskan\u00E9 znalosti o genomu plazmid\u016F pou\u017E\u00EDt pro v\u00FDvoj vylep\u0161en\u00E9 detek\u010Dn\u00ED metody fytoplazem zalo\u017Een\u00E9 na specifick\u00E9 amplifikaci plazmidov\u00E9ho genu a na hybridizaci s univerz\u00E1ln\u00ED sondou v mikroarrays."@cs . . . .