. "Zjistit \u00FAlohu komplex\u016F jadern\u00E9ho aktinu, myosinu a PML v\u00A0genov\u00E9 expresi a tvorb\u011B architektury j\u00E1dra. Charakterizovat proteinov\u00E9 komplexy tubulin\u016F a objasnit jejich roli v organizaci mitotick\u00E9ho apar\u00E1tu, interakc\u00EDch s\u00A0membr\u00E1nami a p\u0159i ur\u010Dov\u00E1n\u00ED polarity bun\u011Bk. Objasnit mechanismus dopravov\u00E1n\u00ED integr\u00E1ln\u00EDch b\u00EDlkovin do plazmatick\u00E9 membr\u00E1ny. Objasnit pod\u00EDl cytoskelet\u00E1ln\u00EDch interakc\u00ED eIF3 transla\u010Dn\u00EDho komplexu na lokalizovan\u00E9 translaci a dynamice stresov\u00FDch granul\u00ED. Objasnit funkci komplexu Isw2-Itc1, kter\u00FD remoduluje chromatin, v\u00A0pr\u016Fb\u011Bhu bun\u011B\u010Dn\u00E9ho cyklu a p\u0159i reakci na stres. Ur\u010Dit topologii protein\u016F v biologick\u00FDch membr\u00E1n\u00E1ch pomoc\u00ED specifick\u00E9ho zna\u010Den\u00ED. Vyvinout metodiku pro charakterizaci vysokomolekul\u00E1rn\u00EDch komplex\u016F s pou\u017Eit\u00EDm elektrospreje na iontov\u00E9m cyklotronu. Identifikovat endocytickou dr\u00E1hu pou\u017E\u00EDvanou polyomaviry pro dopravu jejich genom\u016F do bun\u011B\u010Dn\u00E9ho j\u00E1dra a obrann\u00E9 bun\u011B\u010Dn\u00E9 mechanismy, sv\u00E1d\u011Bj\u00EDc\u00ED virov\u00E9 \u010D\u00E1stice na degrada\u010Dn\u00ED dr\u00E1hy. Porozum\u011Bt mechanismu morfogenese virion\u016F a identifikovat bun\u011B\u010Dn\u00E9 funkce, kter\u00E9 se tohoto procesu \u00FA\u010Dastn\u00ED."@cs . . "Centrum funk\u010Dn\u00ED organizace bu\u0148ky"@cs . "3"^^ . . . . "96589"^^ . . . . . "104"^^ . "cell, nucleus, cytoskeleton, compartmentalization, cellular trafficking, gene expression, virus infection, cell cycle, cellular senescence"@en . "2011-12-31+01:00"^^ . . "104"^^ . "Functional Organisation of the Cell"@en . . "0"^^ . . "131084"^^ . "Establishing the roles of complexes of nuclear actin, myosin and PML in gene expression and formation of nuclear architecture. Characterization of protein complexes of tubulins and their roles in organization of mitotic apparatus, interactions with membranes and establishing cellular polarity. Determination of transport mechanism of integral proteins towards the cell membrane. To clarify how cytoskeletal interactions of eIF3 translation complex participate in localized translation and dynamics of stress granules. To determine the function of chromatin remodeller Isw2-Itc1 in the cell cycle and stress. Description of protein topology in biological membranes using specific markers. Technique development for characterization of protein complexes using electrospray on ionic cyclotron. To identify the endocytotic pathway used by polyomaviruses for transporting their genomes to the cell nucleus, and the cellular protective mechanisms leading the virus to degradation pathways. To describe cellular structures involved in virion morphogenesis."@en . "2005-02-01+01:00"^^ . "1"^^ .