. "1"^^ . . "1072"^^ . . "1"^^ . . . "Selekce scFv protil\u00E1tek proti rekombinantn\u00EDmu LsaA proteinu bakterie Lawsonia intracellularis"@cs . . "Neuvedeno."@en . . "\"The goal of the project is the selection and characterization of recombinant scFv antibodies against LsaA protein of Lawsonia intracellularis. The gene coding LsaA protein will be cloned into the plasmid vector and corresponding protein will be then expressed with a suitable fusion partner enabling its expression in soluble form and efficient purification. Recombinant protein will be purified and used for scFv selection and construction of immunoenzymatic test for Lawsonia intracellularis serological detection. ScFv antibodies will be selected using \"\"Human Synthetic VH + VL scFv Library\"\" (Griffin.1 Library). Selected cloneswill be tested for their ability to bind native bacterial LsA protein, sequenced and their affinity constant will be determined.We plan to use these antibodies in immunohistochemical detection of bacteria in tissue sections and for the construction of immunoenzymatic tests.\""@en . "\"C\u00EDlem projektu je selekce a charakteristika rekombinantn\u00EDch scFv protil\u00E1tek proti povrchov\u00E9mu proteinu LsaA bakterieLawsonia intracellularis. Tento c\u00EDl bude uskute\u010Dn\u011Bn klonov\u00E1n\u00EDm \u010D\u00E1sti genomu bakterie, k\u00F3duj\u00EDc\u00ED povrchov\u00FD LsaA rekombinantn\u00EDm partnerem umo\u017E\u0148uj\u00EDc\u00EDm jeho solubiln\u00ED expresi a n\u00E1slednou purifikaci. Z\u00EDskan\u00FD protein bude p\u0159\u00EDslu\u0161n\u00FDm zp\u016Fsobem purifikov\u00E1n a vyu\u017Eit p\u0159i konstrukci imunoenzymatick\u00FDch test\u016F k diagnostice bakterie. Hlavn\u00ED aplikac\u00ED z\u00EDskan\u00E9ho LsaA proteinu bude selekce rekombinantn\u00EDch scFv protil\u00E1tek. Tyto protil\u00E1tky budou selektov\u00E1ny z \"\"Human Synthetic VH + VL scFv Library\"\" (Griffin.1 Library). Reaguj\u00EDc\u00ED klony budou testov\u00E1ny na schopnost vazby na nativn\u00ED bakteri\u00E1ln\u00ED protein a d\u00E1le budou charakterizov\u00E1ny sekvenov\u00E1n\u00EDm a stanoven\u00EDm jejich afinitn\u00ED konstanty. P\u0159edpokl\u00E1d\u00E1me praktick\u00E9 vyu\u017Eit\u00ED t\u011Bchto protil\u00E1tek v imunohistochemick\u00E9m pr\u016Fkazu bakterie a konstrukci nov\u00FDch typ\u016F diagnostick\u00FDch test\u016F.\""@cs . . . "1"^^ . "The selection of scFv antibodies against recombinant LsaA protein of Lawsonia intracellularis"@en . "0"^^ . . "0"^^ . "1072"^^ .