. "0"^^ . "2006-01-01+01:00"^^ . "3"^^ . . . . . . "This project is designed to study possible mechanisms of anti-inflammatory effects and to detect side effects of\u00A0sanguinarine, chelerythrine, berberine, protopine, and colchicine,\u00A0all alkaloids displaying anti-inflammatory activity. The project is focused on studying the influence of tested alkaloids on the expression of enzymes whose activity is necessary for the involvement of neutrophilic granulocytes in inflammatory response. As a well-established model for studying phagocyte NADPH oxidase, myeloperoxidase (MPO), and cyclooxygenase 2 (COX-2), human promyelocytic leukemia HL-60 cells induced by dimethyl sulfoxide to\u00A0differentiate into neutrophil-like cells will be used. The aim of the designed experiments is to explore the effect of tested alkaloids on induction of apoptosis, the\u00A0effect on expression of\u00A0selected NADPH oxidase subunits, i.e. membrane subunit gp91phox and cytosolic subunit p67phox, and the effect on expression and activity of MPO and COX-2 during differentiation of HL-60"@en . "3"^^ . "1044"^^ . . "NADPH oxidase; myeloperoxidase; cyclooxygenase 2; isoquinoline alkaloids; colchicine; HL-60"@en . . "Changes of expression of pro-inflammatory enzymes caused by isoquinoline alkaloids during differentiation of promyelocytes into neutrophils"@en . . "0"^^ . . "Z\u00E1m\u011Brem p\u0159edkl\u00E1dan\u00E9ho projektu je\u00A0studium mo\u017En\u00FDch mechanism\u016F\u00A0protiz\u00E1n\u011Btliv\u00FDch \u00FA\u010Dink\u016F a z\u00EDsk\u00E1n\u00ED nov\u00FDch poznatk\u016F o vedlej\u0161\u00EDch \u00FA\u010Dinc\u00EDch\u00A0sanguinarinu, chelerythrinu, berberinu, protopinu a kolchicinu, alkaloid\u016F vykazuj\u00EDc\u00EDch protiz\u00E1n\u011Btlivou aktivitu. Projekt je soust\u0159ed\u011Bn na studium vlivu vybran\u00FDch alkaloid\u016F na\u00A0expresi enzym\u016F,\u00A0jejich\u017E aktivita je nezbytn\u00E1 pro zapojen\u00ED neutrofiln\u00EDch granulocyt\u016F do z\u00E1n\u011Btliv\u00E9 reakce, p\u0159i\u010Dem\u017E jako vhodn\u00FD model pro studium fagocytov\u00E9 NADPH-oxidasy, myeloperoxidasy (MPO) a cyklooxygenasy 2 (COX-2)\u00A0budou vyu\u017E\u00EDv\u00E1ny bu\u0148ky lidsk\u00E9 promyelocytov\u00E9 leukemie HL-60 diferencuj\u00EDc\u00ED vlivem\u00A0dimethylsulfoxidu na bu\u0148ky neutrofiln\u00EDho typu.\u00A0P\u0159i \u0159e\u0161en\u00ED\u00A0projektu bude b\u011Bhem diferenciace bun\u011Bk HL-60 studov\u00E1n vliv alkaloid\u016F na indukci apoptosy, vliv alkaloid\u016F na expresi vybran\u00FDch\u00A0podjednotek NADPH-oxidasy, tj. membr\u00E1nov\u00E9 podjednotky gp91phox a cytosolov\u00E9 podjednotky\u00A0p67phox,\u00A0vliv na\u00A0expresi a aktivitu MPO a COX-2 a d\u00E1le budou\u00A0studov\u00E1ny interakce alkaloid\u016F se sign\u00E1ln\u00ED dr\u00E1hou\u00A0nukle\u00E1rn\u00EDho"@cs . "2008-12-31+01:00"^^ . "Zm\u011Bny exprese proz\u00E1n\u011Btliv\u00FDch enzym\u016F vyvolan\u00E9 isochinolinov\u00FDmi alkaloidy v pr\u016Fb\u011Bhu diferenciace promyelocyt\u016F na neutrofily"@cs . . "1044"^^ . . "1"^^ .