"0"^^ . "3"^^ . . . "Betulinic acid derivatives, identification of mechanism of anticancer action based on expression profiling."@en . "C\u00EDlem projektu je uskute\u010Dnit expresn\u00ED anal\u00FDzu \u0161esti cytotoxick\u00FDch deriv\u00E1t\u016F kyseliny betulinov\u00E9 na n\u00E1dorov\u00E9 linii CEM. Expresn\u00ED profily budou porovn\u00E1ny s profily sandardn\u00EDch l\u00E9\u010Div, diferen\u010Dn\u00ED data v\u00FDznamn\u011B p\u0159isp\u011Bj\u00ED k pochopen\u00ED mechanismu \u00FA\u010Dinku a povedou k dal\u0161\u00ED validaci terapeutick\u00FDch c\u00EDl\u016F."@cs . "1"^^ . . "Czech Republic is a country with a relatively\u00A0high rate of cancer. The\u00A0unsatisfactory results of anticancer therapy to date\u00A0and\u00A0the narrow spectrum of targeted drugs require active search for compounds with novel mechanisms of antitumour action. Our group is studying the mechanisms\u00A0by which the triterpenoid compounds exert their cytotoxic effects. The most potent of these\u00A0compounds induced apoptosis in a concentration less than IC50=0,2mM. We have identified cytochrome c as one unique target of b-keto betulinic acid. However, other potent compounds reveal a\u00A0different kinetic of apoptosis induction with shifts in cell cycle distribution.\u00A0Owing to the\u00A0wide spectrum of possible targets we\u00A0plan to use expression fingerprinting (profiling of mRNA/proteins) of a treated\u00A0CEM cancer cell line.\u00A0The\u00A0expression profiles obtained\u00A0will be compared\u00A0to the\u00A0profiles of drugs with identified mechanisms of action and further statistically analyzed.\u00A0The differential data is expected to contribute to our\u00A0understanding of the mechanism of action and facilitate target validation of triterpenoid compounds."@en . "1167"^^ . . . . . "Deriv\u00E1ty kyseliny betulinov\u00E9, identifikace mechanism\u016F protin\u00E1dorov\u00E9ho \u00FA\u010Dinku pomoc\u00ED expresn\u00EDho profilov\u00E1n\u00ED."@cs . . "mechanismus \u00FA\u010Dinku, cytotoxicita, protin\u00E1dorov\u00E1 terapie, betulinov\u00E1 kyselina"@en . "2009-01-01+01:00"^^ . "2011-12-31+01:00"^^ . . . . "0"^^ . "1167"^^ . "3"^^ . .