"\"The trypanosomatid SL RNA plays an essential role in trans-splicing and processing of all mRNA. SL RNA provides the m7G-capped SL sequence to the 5'end of every mRNA. The cap structure of the SL RNA is unique in eukaryotes with 4 nucleotides after the m7G cap carrying a total of seven methyl groups and by convention is referred to as \"\"cap 4\"\". The RNA methyltransferases that catalyze cap 4 modifications are unique to trypanosomatids. It is likely, that the enzymes involved in cap 4 formation, play a pivotal role in regulating the availability of trans-splicing \"\"competent\"\" SL RNA and, therefore, these enzymes might be major players in regulating trans-splicing activity as a whole. Recently, several cap 4 modifying enzymes have been characterized. Theaim of this project is to analyze the function of potential SL RNA methyltransferase MT420 in Trypanosoma brucei. The specific aims of the project involve investigation of a role of MT420 for SL RNA biogenesis and trans-splicing, determination its\""@en . "2008-01-01+01:00"^^ . "\"SL RNA u trypanosomatid m\u00E1 v\u00FDznamnou roli p\u0159i \"\"trans-sest\u0159ihu\"\" a\u00A0 \u00FAprav\u00E1ch mRNA. SL RNA poskytuje ka\u017Ed\u00E9 mRNA tzv. SL sekvenci. SL sekvence je kr\u00E1tk\u00FD \u00FAsek SL RNA charakterizovan\u00FD m7G \u010Depi\u010Dkou na 5\u00B4-konci n\u00E1sledovanou 4 nukleotidy nesouc\u00EDmi sedm methylov\u00FDch skupin. Tato SL RNA \u010Depi\u010Dka je ozna\u010Dov\u00E1na jako \"\"cap 4\"\" a je mezi eukaryotick\u00FDmi organismy jedine\u010Dn\u00E1. RNA methyltransfer\u00E1zy, kter\u00E9 katalyzuj\u00ED modifikace SL sekvence u trypanosom jsou rovn\u011B\u017E unik\u00E1tn\u00ED. Je pravd\u011Bpodobn\u00E9, \u017Ee tyto enzymy, \u00FA\u010Dastn\u00EDc\u00EDse modifikace SL sekvence, hraj\u00ED hlavn\u00ED roli v\u00A0regulaci dostupnosti SL RNA pro \"\"trans-sest\u0159ih\"\" a mohou tak b\u00FDt d\u016Fle\u017Eit\u00FDmi akt\u00E9ry v\u00A0regulaci tohoto z\u00E1sadn\u00EDho procesu. B\u011Bhem posledn\u00EDch let bylo charakterizov\u00E1no n\u011Bkolik enzym\u016F \u00FA\u010Dastn\u00EDc\u00EDch se modifikace \u010Depi\u010Dky SL RNA. C\u00EDlem tohoto projektu je analyzovat funkci potenci\u00E1ln\u00ED methyltransfer\u00E1zy MT420 \u00FA\u010Dastn\u00EDc\u00ED se modifikace SL sekvence u Trypanosoma brucei - v\u00FDznamn\u00E9ho parazitick\u00E9ho prvoka savc\u016F.\""@cs . "1"^^ . "1035"^^ . "1035"^^ . . . . . . "2010-12-31+01:00"^^ . "Funk\u010Dn\u00ED anal\u00FDza methyltransfer\u00E1zy \u00FA\u010Dastn\u00EDc\u00ED se modifikace SL RNA u Trypanosoma brucei"@cs . . . . . "1"^^ . "T. brucei; methyltranserases; SL RNA; trans-splicing; cap structure; RNA interference"@en . . "0"^^ . "0"^^ . . "1"^^ . "Functional analysis of methyltransferase involved in SL RNA modification in Trypanosoma brucei"@en .