. "1"^^ . . "2008-01-01+01:00"^^ . "2"^^ . "32"^^ . . "32"^^ . . . . "Synbiotics and plant extracts in human and animal nutrition"@en . . . . "probiotika; prebiotika; synbiotika; rostlinn\u00E9 extrakty; mastn\u00E9 kyseliny"@en . "Vyu\u017Eit\u00ED synbiotik a rostlinn\u00FDch extrakt\u016F ve v\u00FD\u017Eiv\u011B lid\u00ED a zv\u00ED\u0159at"@cs . "5344"^^ . "0"^^ . "5344"^^ . . "The first aim of the project will be testing of probiotics, prebiotics and synbiotics in human and animal nutrition. The impact of plant extract on intestinal flora and on pure culture of intestinal bacteria will also be studied. Bifidobacteria will be studied as a most frequent and important probiotics bacterial. Bifidobacterial counts in human and animal faecal samples will be determined. Pure culture of new bifidobacterial strains will be isolated, identified and characterized. Complex intestinal flora composition will also be studied using FISH, PCR, PCR-RAPD, RT-PCR and DGGE techniques. Plant extract will be tested for prebiotics effects (e.g. extracts with high inulin and oligofructans contents) Antibacterial properties of selected plant extracts will also be tested. TLC, HPLC and GC will be used for the identification of effective compounds. Medium-chain fatty acids and their glycerides belong to efficient antimicrobial agents of plant origin as well. Experiments with these compounds will be aimed at inhibition of growth of\u00A0 Campylobacter jejuni."@en . "C\u00EDlem projektu je selekce nov\u00FDch kmen\u016F probiotick\u00FDch bakteri\u00ED, zejm\u00E9na na b\u00E1zi bifidobakteri\u00ED, vhodn\u00FDch k pou\u017Eit\u00ED spole\u010Dn\u011B s prebiotiky a rostlinn\u00FDmi extrakty ve v\u00FD\u017Eiv\u011B lid\u00ED a zv\u00ED\u0159at. C\u00EDlem je d\u00E1le nal\u00E9zt rostlinn\u00E9 extrakty a mastn\u00E9 kyseliny s antimikrobi\u00E1ln\u00EDmi \u00FA\u010Dinky proti aliment\u00E1rn\u00EDm patogen\u016Fm."@cs . "2011-12-31+01:00"^^ . .