. . "4"^^ . "Center of novel approaches to bioanalysis and molecular diagnostics"@en . "84326"^^ . . . . "Projekt je zam\u011B\u0159en na navr\u017Een\u00ED nov\u00FDch technik molekul\u00E1rn\u00ED diagnostiky, zalo\u017Een\u00FDch p\u0159edev\u0161\u00EDm na elektrochemick\u00E9 anal\u00FDze a principech elektrochemick\u00FDch (bio)senzor\u016F. Hlavn\u00EDm c\u00EDlem projektu je vypracovat nov\u00E9 metody anal\u00FDzy nukleotidov\u00FDch sekvenc\u00ED, mutac\u00ED, monitorov\u00E1n\u00ED genov\u00E9 exprese, DNA-protein interakc\u00ED, stanoven\u00ED genotoxick\u00FDch l\u00E1tek, l\u00E9\u010Div aj. Dosa\u017Een\u00ED tohoto c\u00EDle vy\u017Eaduje v\u00FDrazn\u011B interdisciplin\u00E1rn\u00ED p\u0159\u00EDstup, kter\u00FD bude zaji\u0161t\u011Bn p\u0159edn\u00EDmi laborato\u0159emi v \u010CR zab\u00FDvaj\u00EDc\u00EDmi se v\u00FDzkumem v oblasti bioelektrochemie, analytick\u00E9 chemie a separace, bioorganick\u00E9 chemie, molekul\u00E1rn\u00ED onkologie a proteomiky. V r\u00E1mci projektu budou vyu\u017Eity r\u016Fzn\u00E9 techniky zna\u010Den\u00ED nukleov\u00FDch kyselin a protein\u016F, v\u010Detn\u011B enzymatick\u00E9 inkorporace modifikovan\u00FDch b\u00E1z\u00ED, chemick\u00E9 modifikace biopolymer\u016F, (imuno)techniky zalo\u017Een\u00E9 na enzymatick\u00E9 produkci elektroaktivn\u00EDch indik\u00E1tor\u016F aj. V po\u010D\u00E1te\u010Dn\u00EDm st\u00E1diu budou pro v\u00FDvoj vyu\u017E\u00EDv\u00E1ny p\u0159edev\u0161\u00EDm modelov\u00E9 oligonukleotidy, peptidy a purifikovan\u00E9 proteiny, zat\u00EDmco v pozd\u011Bj\u0161\u00ED f\u00E1zi bude v\u00FDzkum zam\u011B\u0159en na optimalizaci vybran\u00FDch metod pro jejich aplikaci na re\u00E1ln\u00FDch biologick\u00FDch modelech."@cs . . "0"^^ . "2018-12-31+01:00"^^ . "84326"^^ . . "diagnostics; electrochemistry; DNA; proteins; electroactive labels; hybridization; mutations"@en . . "The project is focused on the design of novel techniques of molecular diagnostics, based primarily on electrochemical analysis and principles of electrochemical (bio)sensors. The main goal of the project is to develop novel techniques for the analysis of nucleotide sequences, mutations, gene expression monitoring, DNA-protein interactions, determination of genotoxic substances, drugs etc. Reaching this goal requires markedly interdisciplinary approach that will be ensured by leading Czech groups involved in bioelectrochemistry, analytical chemistry and separation, bioorganic chemistry, molecular oncology and proteomics. Within the project, various techniques of nucleic acids and protein labelling will be applied, including enzymatic incorporation of modified bases, chemical modification of biopolymers, enzyme linked (immuno)assays etc. In initial stages, mainly model oligonucleotides, peptides and purified protein will be utilized, while in the later stages the research will be focused on the optimalization of selected techniques for their application on real biological models."@en . . "29"^^ . "1"^^ . . . . "29"^^ . "Centrum nov\u00FDch p\u0159\u00EDstup\u016F k bioanal\u00FDze a molekul\u00E1rn\u00ED diagnostice"@cs . "2012-01-01+01:00"^^ .