. . "0"^^ . "antimicrobial peptides; Toll-like receptors; ontogeny; bacterial infection; pig"@en . . "2009-01-01+01:00"^^ . "Antimikrobi\u00E1ln\u00ED peptidy a Toll-like receptory v ontogenezi a p\u0159i bakteri\u00E1ln\u00EDch infekc\u00EDch"@cs . "0"^^ . "1"^^ . . "5"^^ . . "5"^^ . "5860"^^ . "5860"^^ . . "Antimicrobial peptides and Toll-like receptors in ontogeny and bacterial infections"@en . "2013-12-31+01:00"^^ . . . . "Antimicrobial peptides (AMPs) are endogeneous substances protecting organisms against infection. Basic knowledge of them and possibility of their induction is needed due to\u00A0 increasing resistance of pathogenic bacteria against classical microbial antibiotics. \u00A0\u00A0\u00A0 Toll-like receptors (TLRs) recognize various microbial substances known as microbial-associated molecular patterns (MAMPs) and their expression and activation is necessary for induction of the natural immune response.\u00A0\u00A0\u00A0\u00A0 The expression of selected AMPs and TLRs will be studied by RT-PCR \u00A0in pig intestine in prenatal and early postnatal ontogeny of the pig. The effect of fetal gut infection (after surgical intervention) and the effect of oral infection of germ-free pigs with E.coli on the expression of AMPs and TLRs will be studied. The model EPEC, a nonpathogenic and a probiotic E.coli strains will be compared. Finally, the role of birth, weaning and endogeneous gut microflora will be studied in conventional pigs."@en . . "Antimikrobi\u00E1ln\u00ED peptidy (AMP) jsou endogenn\u00ED substance chr\u00E1n\u00EDc\u00ED organismus proti infekci. Neust\u00E1l\u00FD n\u00E1r\u016Fst rezistence mikrob\u016F proti klasick\u00FDm antibiotik\u016Fm vyvolal nal\u00E9havou pot\u0159ebu studovat tato endogenn\u00ED antibiotika s\u00A0c\u00EDlem zvy\u0161ovat jejich produkci.\u00A0\u00A0\u00A0 Toll-like receptory (TLR) rozezn\u00E1vaj\u00ED r\u016Fzn\u00E9 mikrobi\u00E1ln\u00ED substance zn\u00E1m\u00E9 jako struktury\u00A0asociovan\u00E9 s mikroby (MAMP) a jejich exprese a aktivace je v\u00FDznamn\u00E1 p\u0159edev\u0161\u00EDm pro indukci p\u0159irozen\u00E9 imunitn\u00ED odpov\u011Bdi. \u00A0\u00A0\u00A0 Exprese vybran\u00FDch AMP a TLR\u00A0 bude studov\u00E1na pomoc\u00ED RT-PCR ve st\u0159ev\u011B prasete v\u00A0prenat\u00E1ln\u00ED a \u010Dasn\u00E9 postnat\u00E1ln\u00ED ontogenezi. Bude studov\u00E1n vliv E.coli infekce \u00A0fet\u00E1ln\u00EDho st\u0159eva (chirurgicky) a vliv peror\u00E1ln\u00ED infekce bezmikrobn\u00EDch selat na expresi AMP a TLR. \u00DA\u010Dinek modelov\u00E9ho EPEC bude srovn\u00E1n s \u00FA\u010Dinkem nepatogenn\u00EDho a probiotick\u00E9ho kmene E.coli. U konven\u010Dn\u00EDch prasat bude sledov\u00E1n vliv porodu, odstavu a st\u0159evn\u00ED mikrofl\u00F3ry. \u00A0\u00A0"@cs . . .