. "2008-12-31+01:00"^^ . "0"^^ . "The main objective of the present project is to testing of hypothesis, that the titre of neutralizing antibodies influences the level of viremia in PCV-2 infected piglets. Viremia and the titer of neutralizing antibodies will be quantified in experimentally and naturally infected piglets by real-time PCR and serological methods. Additional objective of this study is to evaluate neutralizing effect of scFv antibodies against capsid protein of porcine circovirus 2 (PCV-2) and to localise neutralization epitopes. We plan to generate scFv antibodies against recombinant capsid protein of virus and to evaluate neutralizing activity of these antibodies by virus titration on tissue cultures. Although neutralizing activity of immune sera was demonstrated in the case of PCV-2 virus, the exact localization of neutralizing epitope(s) was never demonstrated. The advantage of using scFv antibodies instead of standard monoclonal antibodies is the shortening of selection procedure (weeks instead of"@en . "1825"^^ . . . . . "Vliv neutraliza\u010Dn\u00EDch protil\u00E1tek na vir\u00E9mii a lokalizace neutraliza\u010Dn\u00EDch epitop\u016F cirkoviru prasat 2"@cs . "neutralisation; antibodies; viremia; epitopes; circovirus"@en . . "0"^^ . "1"^^ . "5"^^ . "1825"^^ . "Hlavn\u00EDm c\u00EDlem projektu je testov\u00E1n\u00ED hypot\u00E9zy, \u017Ee titr neutraliza\u010Dn\u00EDch protil\u00E1tek ovliv\u0148uje \u00FArove\u0148 viremie u PCV-2 infikovan\u00FDch selat. Viremie i titr neutraliza\u010Dn\u00EDch protil\u00E1tek bude kvantifikov\u00E1na na experiment\u00E1ln\u011B i p\u0159irozen\u00FDm zp\u016Fsobem infikovan\u00FDch selatech pomoc\u00ED real-time PCR a s\u00E9rologick\u00FDch test\u016F. Dal\u0161\u00EDm c\u00EDlem projektu je p\u0159esn\u00E1 lokalizace neutraliza\u010Dn\u00EDch epitop\u016F na povrchu proteinu kapsidy circoviru prasat 2 (PCV-2). Uskute\u010Dn\u00EDme selekci scFv protil\u00E1tek proti rekombinantn\u00EDmu proteinu kapsidy viru a stanov\u00EDme jejich neutraliza\u010Dn\u00ED aktivitu titrac\u00ED na bun\u011B\u010Dn\u00FDch kultur\u00E1ch. A\u010Dkoliv neutralizace viru protil\u00E1tkami byla ji\u017E u PCV-2 viru prok\u00E1z\u00E1na, p\u0159esn\u00E1 lokalizace neutraliza\u010Dn\u00EDch epitop\u016F nebyla zat\u00EDm stanovena. V\u00FDhoda pou\u017Eit\u00ED scFv protil\u00E1tek ve srovn\u00E1n\u00ED s klasick\u00FDmi monoklon\u00E1ln\u00EDmi protil\u00E1tkami je ve zkr\u00E1cen\u00ED doby pott\u0159ebn\u00E9 pro selekci (t\u00FDdny m\u00EDsto m\u011Bs\u00EDc\u016F), v mo\u017Enosti ovliv\u0148ov\u00E1n\u00ED afinity vazby t\u011Bchto molekul a jejich perspektiv\u00EDho vyu\u017Eit\u00ED v diagnostice a p\u0159\u00EDpadn\u011B terapii onemocn\u011Bn\u00ED. P\u0159edkl\u00E1dan\u00FD"@cs . . . "2006-01-01+01:00"^^ . . "The control of viremia by neutralizing antibodies and localization of neutralization epitopes of porcine circovirus 2"@en . "5"^^ . . . .