. . . "Brachyspira hyodysenteriae je p\u016Fvodcem dysenterie prasat (DP),\u00A0 proti kter\u00E9 se doposud nepoda\u0159ilo p\u0159ipravit spolehlivou vakc\u00EDnu.\u00A0 Tlumen\u00ED DP se omezuje na antibiotickou terapii, co\u017E je vzhledem k vzr\u016Fstaj\u00EDc\u00ED rezistenci do budoucna nep\u0159ijateln\u00E9. Nejz\u00E1va\u017En\u011Bj\u0161\u00EDm zdrojem \u0161\u00ED\u0159en\u00ED DP mezi st\u00E1dy jsou zv\u00ED\u0159ata s asymptomn\u00ED infekc\u00ED B. hyodysenteriae. K\u00A0jejich odhalov\u00E1n\u00ED \u00A0se jev\u00ED jako \u00FA\u010Deln\u00E9 vyu\u017Eit kombinace metod p\u0159\u00EDm\u00E9ho pr\u016Fkazu (kultivace, DNA metody) \u00A0s\u00A0dostate\u010Dn\u011B citliv\u00FDmi metodami pro pr\u016Fkaz druhov\u011B specifick\u00FDch protil\u00E1tek, ty v\u0161ak nejsou dosud komer\u010Dn\u011B dostupn\u00E9. Ned\u00E1vno identifikovan\u00FD a klonovan\u00FD lipoprotein BmpB, kter\u00FD je specifick\u00FD pro vn\u011Bj\u0161\u00ED membr\u00E1nu B. hyodysenteriae, bude vyu\u017Eit k v\u00FDvoji s\u00E9rologick\u00FDch test\u016F. Tak\u00E9 rekombinantn\u00ED protil\u00E1tky protidruhov\u011B specifick\u00FDm epitop\u016Fm lipoproteinu BmpB budou uplatn\u011Bny v\u00A0diagnostice DP. P\u0159edm\u011Btem tohoto projektu je p\u0159\u00EDprava a\u00A0vyu\u017Eit\u00ED rekombinantn\u00EDho lipoproteinu BmpB a scFv protil\u00E1tek v\u00A0diagnostice DP, zejm\u00E9na k pr\u016Fkazu asymptomn\u00EDch infekc\u00ED."@cs . . "0"^^ . "Recombinant lipoprotein (BmpB) of the outer membrane of B. hyodysenteriae and scFv antibodies as a means of determination of asymptomatic carriers"@en . . "Brachyspira hyodysenteriae is the causative agent of swine dysentery (SD), which remains an important endemic disease in many pig-rearing countries, where control is limited by a lack of effective vaccines and by the emergence of spirochaete strains withreduced susceptibility to antimicrobials. A rational solution is offered by a gradual elimination of positive herds, protection of infection-free herds, and the establishment of SPF herds of pigs.\u00A0 The main goal of the project is a comprehensive approachto the identification of asymptomatic carriers of B. hyodysenteriae. \u00A0To attain the goal, the recombinant BmpB protein and specific recombinant antibodies against epitopes of the lipoprotein BmpB specific for B. hyodysenteriae will be prepared. \u00A0Serological tests developed will be used for a study of the dynamics of production of specific antibodies in experimentally and naturally infected pigs."@en . "5"^^ . . "0"^^ . "5"^^ . . "pig; swine dysentery; serologic techniques"@en . "2008-12-31+01:00"^^ . "1"^^ . "2006-01-01+01:00"^^ . . "2325"^^ . "2325"^^ . "Rekombinantn\u00ED lipoprotein (BmpB) vn\u011Bj\u0161\u00ED membr\u00E1ny Brachyspira hyodysenteriae a scFv protil\u00E1tky jako prost\u0159edek pr\u016Fkazu asymptomn\u00EDch infekc\u00ED prasat"@cs . . . .