"0"^^ . "Recently has been proposed that certain chemicals in the environment (fytoestrogens, pesticides etc.) and medical products (e.g. antibiotics) may mimic or inhibit actions of natural hormones or alter the normal regulatory function of the endocrine system. Due to complexity of mechanisms involved in the tissue responsiveness to these compounds a powerful in vivo test(s) has to be used to evaluate the entire complexity of a possible response. In this project such a model system for evaluation of steroid hormone agonist and antagonist activities of different compounds will be established in mice by simultaneous evaluation of responses on the mammary gland, uterus, seminal vesicles and spleen in young intact and adult gonadectomized mice of C3H strain. The role of IGF system and G protein in the mechanism of action some estrogens, progestins, androgens and their antagonists will be determined in vivo and in vitro (whole organ culture and organ culture of the mammary glands) by means of western"@en . . . "Animal model for testing steroid hormone agonists and antagonists and role of IGF syst\u00E9m and G proteins in the mechanism of steroid hormone actions"@en . "4"^^ . . "0"^^ . "4"^^ . . . . "1"^^ . . . . "Ned\u00E1vno bylo p\u0159edlo\u017Eeno, \u017Ee ur\u010Dit\u00E9 chemick\u00E9 l\u00E1tky v prost\u0159ed\u00ED (fytoestrogeny, pesticidy atd.) a farmaceutick\u00E9 produkty (nap\u0159. antibiotika) mohou mimikovat nebo inhibovat p\u016Fsoben\u00ED p\u0159irozen\u00FDch hormon\u016F nebo naru\u0161ovat norm\u00E1ln\u00ED regula\u010Dn\u00ED funkci endokrinn\u00EDho syst\u00E9mu. V d\u016Fsledku komplexnosti mechanism\u016F \u0159\u00EDd\u00EDc\u00EDch reakceschopnost tk\u00E1n\u00ED k t\u011Bmto l\u00E1tk\u00E1m spolehliv\u00FD a v\u00FDkonn\u00FD in vivo test (testy) mus\u00ED b\u00FDt pou\u017Eit k vyhodnocen\u00ED cel\u00E9ho komplexu mo\u017En\u00FDch \u00FA\u010Dink\u016F. V r\u00E1mci \u0159e\u0161en\u00ED tohoto projektu takov\u00FD model pro hodnocen\u00ED biologick\u00FDch aktivit l\u00E1tek s \u00FA\u010Dinky agonist\u016F a antagonist\u016F steroidn\u00EDch hormon\u016F bude vypracov\u00E1n na my\u0161\u00EDch. Simult\u00E1nn\u011B budou hodnoceny \u00FA\u010Dinky t\u011Bchto l\u00E1tek na ml\u00E9\u010Dn\u00E9 \u017El\u00E1ze, d\u011Bloze, semenn\u00FDch v\u00E1\u010Dc\u00EDch a slezin\u011B u mlad\u00FDch intaktn\u00EDch a dosp\u011Bl\u00FDch gonadektomizovan\u00FDch my\u0161\u00EDch kmene C3H. \u00DAloha IGF syst\u00E9mu a G protein\u016F v mechanismu \u00FA\u010Dinku l\u00E1tek s \u00FA\u010Dinky agonist\u016F estrogen\u016F, gestagen\u016F, androgen\u016F a jejich antagonist\u016F bude stanovena in vivo a in vitro (celoorg\u00E1nov\u00E1 a org\u00E1nov\u00E1 kultura ml\u00E9\u010Dn\u00E9 \u017El\u00E1zy) pomoc\u00ED"@cs . "Model pro testov\u00E1n\u00ED agonist\u016F a antagonist\u016F steroidn\u00EDch hormon\u016F in vivo a \u00FAlohy IGF syst\u00E9mu a G protein\u016F v mechanismu p\u016Fsoben\u00ED steroidn\u00EDch hormon\u016F"@cs . "1162"^^ . . "Neuvedeno."@en . "3265"^^ . .