"Metodick\u00E9 centrum pro vyu\u017Eit\u00ED molekul\u00E1rn\u00EDch metod v cytogenetice"@cs . . . . "1"^^ . . "Methodical centre for the application of molecular methods io cytogenesis"@en . "5657"^^ . . "Advancement and application of laser microdissection of chromosomes with the following objectives: (1) Construction of bovine and porcine DNA libraries specific for individual chromosomes using the whole-chromosome microdissection. Preparation of painting DNA probes and their use in the catogenetic diagnostics of chromosomal structural aberrations using the multicolour FISH. (2) Construction of region-specific genomic DNA libraries to obtain chromosome-specific probes for the studies of aneuploidy in gametes and early embryos of farm animals. (3) Studies and physical mapping of genomic regions containing genes relevant to performance and health by isolation of clones specific for defined chromosomal regions and preparation of polymorphic microsatelliteprobes. (4) Use of the micro-FISCH technique for the identification of cryptic aberrations, such as derivative chromosomes, ring chromosomes, markers, homogeneously staining regions (HSR) and double minutes (dm)."@en . . . . "0"^^ . "0"^^ . . "0"^^ . "5657"^^ . . "0"^^ . . "Rozvoj laserov\u00E9 mikrodisekce chromozom\u016F a jej\u00ED vyu\u017Eit\u00ED k d\u00E1le uveden\u00FDm c\u00EDl\u016Fm: 1) Vytvo\u0159en\u00ED DNA knihoven, specifick\u00FDch pro jednotliv\u00E9 chromoz\u00F3my skotu a prasat pomoc\u00ED celochromozomov\u00E9 mikrodisekce. P\u0159\u00EDprava malovac\u00EDch DNA sond a jejich vyu\u017Eit\u00ED pro cytogennetickou diagnostiku chromozom\u00E1ln\u00EDch p\u0159estaveb pomoc\u00ED v\u00EDcebarevn\u00E9 FISH. 2) Konstrukce DNA knihoven z mikrodisektovan\u00FDch \u010D\u00E1st\u00ED chromoz\u00F3m\u016F pro z\u00EDsk\u00E1n\u00ED chromozom\u00E1ln\u011B specifick\u00FDch sond pou\u017Eiteln\u00FDch pro studium aneuploidi\u00ED v pohlavn\u00EDch bu\u0148k\u00E1ch a v ran\u00FDch embry\u00EDch hospod\u00E1\u0159sk\u00FDch zv\u00ED\u0159at. 3) Studium a fyzik\u00E1ln\u00ED mapov\u00E1n\u00ED oblast\u00ED genomu s ekonomicky nebo zdravotn\u011B v\u00FDznamn\u00FDmi geny pomoc\u00ED izolace klon\u016F, specifick\u00FDch pro ur\u010Dit\u00E9 oblasti chromoz\u00F3m\u016F a p\u0159\u00EDpravy polymorfn\u00EDch mikrosatelitov\u00FDch sond, specifick\u00FDch pro ur\u010Dit\u00E9oblasti. 4) Vyu\u017Eit\u00ED mikro-FISH techniky pro identifikaci skryt\u00FDch aberac\u00ED jako jsou derivovan\u00E9 chromoz\u00F3my, ring chromoz\u00F3my, marker chromoz\u00F3my, homogenn\u011B se barv\u00EDc\u00ED oblasti (HSR) a double minutes (dm)."@cs .