"1"^^ . . . "Sequencing and the genome analysis of the strawberry vein banding virus"@en . "284"^^ . "12"^^ . . . "284"^^ . "12"^^ . "0"^^ . . "The project will bring original knowledge about the sequence and genome arrangement of the Strawberry vein banding virus (SVBV), which is a new virus recently found in the Europe. The virus will be isolated from infected strawberry plants, where the presence of SVBV will be confirmed by S-blotting. Viral nucleic acid will be isolated and fragmented by restriction endonucleases, cloned into plasmid following standard producers and sequenced. Sequence and the genome arrangement of the SVBV will be analysed and compared with other caulimoviruses. Its sequence will be screened and specific primers for routine diagnosis of the virus by PCR will be prepared. Promoter directed the synthesis of the whole virus genome transcript will be selected from the bank of clones and used in artificial construction to control the expression of \u00DF-glucuronidase (GUS) marker gene."@en . . . "Sekvenov\u00E1n\u00ED a anal\u00FDza genomu viru lemov\u00E1n\u00ED \u017Eilek jahodn\u00EDku"@cs . . "0"^^ . . . "Projekt m\u00E1 p\u0159in\u00E9st nov\u00E9 poznatky o sekvenci, uspo\u0159\u00E1d\u00E1n\u00ED genomu a jeho regula\u010Dn\u00EDch element\u016F v Evrop\u011B nov\u011B popsan\u00E9ho viru lemov\u00E1n\u00ED \u017Eilek jahodn\u00EDku (Strawberry vein banding virus). Virus bude izolov\u00E1n z infikovan\u00FDch rostlin jahodn\u00EDku, ve kter\u00FDch bude p\u0159\u00EDtomnost SVBV potvrzena S-blottingem. Virov\u00E1 DNA bude standardn\u00EDmi metodami fragmentov\u00E1na, klonov\u00E1na do pBS plasmidu a sekvenov\u00E1na. Z\u00EDskan\u00E1 sekvence bude analyzov\u00E1na, porovn\u00E1na se sekvencemi jin\u00FDch caulimovir\u016F a budou podle n\u00ED p\u0159ipraveny specifick\u00E9 p\u00FArim\u00E9ry pro rutinn\u00ED diagnostiku viru metodou PCR. Z vytvo\u0159en\u00E9 banky klon\u016F bude vybr\u00E1n klon obsahuj\u00EDc\u00ED virov\u00FD promotor \u0159\u00EDd\u00EDc\u00ED synt\u00E9zu genomov\u00E9 mRNA. Tento promotor bude upraven restrik\u010Dn\u00EDm \u0161t\u011Bpen\u00EDm a za\u010Dlen\u011Bn do plasmidu, kde bude \u0159\u00EDdit expresi sign\u00E1ln\u00EDho genu beta-glucuronid\u00E1zy (GUS)."@cs . .