. "1"^^ . . "4"^^ . "1"^^ . "3176"^^ . . "Neuvedeno."@en . . . "4"^^ . "The aim of this study is to extend the knowledge concerning the relations between biological properties and genome sequences of Potato mop-top virus (PMTV). Based on complete nucleotide and amino acid sequences the prediction of 3D protein structure and phylogenetic analysis of PMTV isolates will be carried out. Simple and reliable RT PCR method for PMTV detection and isolate classification. will be worked out by designing primers for all three RNAs. In order to get more details concerning molecular biological properties of PMTV infection, protein-protein and protein-RNA interactions will be studied The expected results should help to obtain more details concerning viral life cycle, mainly on complex interaction arising between different viral proteins, of RNA and proteins, of even viral proteins and some host cell proteins known to take part in response to virus infection. The insight into these processes could help to better control of this economically important cell parasite."@en . . "Molekul\u00E1rn\u011B biologick\u00E1 charakterizace, detekce a funk\u010Dn\u00ED anal\u00FDza mop-top viru bramboru (PMTV)"@cs . "Molecular biological characterisation, detection and functional analysis of potato mop-top virus (PMTV)"@en . "0"^^ . . "Podstatou p\u0159edkl\u00E1dan\u00E9ho projektu je bli\u017E\u0161\u00ED charakterizace molekul\u00E1rn\u011B biologick\u00FDch vlastnost\u00ED mop-top viru bramboru (PMTV). Na z\u00E1klad\u011B kompletn\u00ED nukleotidov\u00E9 a aminokyselinov\u00E9 sekvence vybran\u00FDch izol\u00E1t\u016F PMTV bude provedena predikce 3D struktury a fylogenetick\u00E1 anal\u00FDza PMTV izol\u00E1t\u016F. D\u00E1le bude vypracov\u00E1na detek\u010Dn\u00ED metoda zalo\u017Een\u00E1 na RT PCR za pou\u017Eit\u00ED p\u0159\u00EDm\u011Br\u016F ze v\u0161ech t\u0159i genomov\u00FDch RNA, \u010D\u00EDm\u017E bude umo\u017En\u011Bna spolehliv\u011Bj\u0161\u00ED detekce a klasifikace izol\u00E1t\u016F tohoto patogenu. Studium interakc\u00ED protein-proteina protein-RNA umo\u017En\u00ED bli\u017E\u0161\u00ED charakterizaci molekul\u00E1rn\u011B biologick\u00FDch vlastnost\u00ED PMTV a napom\u016F\u017Ee k lep\u0161\u00EDmu porozum\u011Bn\u00ED a t\u00EDm i ke kontrole tohoto ekonomicky d\u016Fle\u017Eit\u00E9ho patogenu. Informace z\u00EDskan\u00E9 \u0159e\u0161en\u00EDm projektu roz\u0161\u00ED\u0159\u00ED z\u00E1kladn\u00ED znalosti genetiky rostlinn\u00FDch vir\u016F a t\u00EDm umo\u017En\u00ED i \u00FA\u010Dinnou ochranu proti t\u011Bmto parazit\u016Fm."@cs . . . "3176"^^ . . .