"Genetick\u00E1 informace eukaryot je ulo\u017Eena v line\u00E1rn\u00EDch struktur\u00E1ch, DNA a chromoz\u00F3mech. Lokalizace gen\u016F na chromozomu m\u00E1 velk\u00FD v\u00FDznam na genovou expresi i replikaci DNA a ostatn\u00ED spojen\u00E9 fyziologick\u00E9 projevy rostlinn\u00E9 bu\u0148ky. Tyto projevy se v\u0161ak uplat\u0148uj\u00EDzejm\u00E9na v interf\u00E1zi. Znalost struktury a funkci interf\u00E1zn\u00EDho j\u00E1dra je z\u00E1sadn\u00EDm p\u0159edpokladem pro porozum\u011Bn\u00ED r\u016Fstov\u00FDm a v\u00FDvinov\u00FDm proces\u016Fm u rostlin. B\u011Bhem na\u0161\u00ED p\u0159edchoz\u00ED pr\u00E1ce jsme vyvinuli imunocytochemick\u00E9 metody, kter\u00FDmi jsme detekovali aktivituchromatinu jednak na mitotick\u00FDch chromoz\u00F3mech, jednak v interf\u00E1zn\u00EDch bun\u011B\u010Dn\u00FDch j\u00E1drech. P\u0159inesli jsme \u0159adu d\u016Fkaz\u016F o korelaci aktivity chromatinu s \u010Dasnou replikac\u00ED chromoz\u00F3mov\u00FDch oblast\u00ED bohat\u00FDch na geny. Vzhledem k tomu, \u017Ee rostliny s r\u016Fzn\u011B velk\u00FDmigenomy maj\u00ED i r\u016Fznou z\u00E1kladn\u00ED strukturu uspo\u0159\u00E1d\u00E1n\u00ED jadern\u00E9ho chromatinu, chceme se v p\u0159edlo\u017Een\u00E9m projektu soust\u0159edit na komparaci dvou model\u016F, Arabidopsis a Silene. Budeme studovat genov\u00E9 lokusy a v\u00FDrazn\u00E9 chromoz\u00F3mov\u00E9 struktury (centrom\u00E9ry, telom\u00E9ry, eu-"@cs . . "1"^^ . . "6924"^^ . . "Neuvedeno."@en . . . "Studium organizace rostlinn\u00E9ho jadern\u00E9ho chromatinu"@cs . "Cytogenetic study of nuclear chromatin organization in plants"@en . "16"^^ . "16"^^ . . . . . "0"^^ . . "Main body of cytogenetic data in flowering plants comes from the observations on mitotic chromosomes. Linear structure of chromosomes (ordered arrangement of genes, heterochromatin domains, location of prominent chromosomal structures as telomeres andcentomeres) has substantial significance on gene expression and DNA replication during interphase. The knowledge of genome organisation and function is a critical point in understanding the physiologiclal processes during plant growth and development. Inour previous work with we elaborated immunocytochemical detection of transcribed chromatin in both, mitotic chromosomes and interphase nuclei and brought some indirect evidence of the correlation of chromatin activity with early replication andlocalisation of the majority of genes. Since plants with small and large genomes have different organisation of chromatin, we propose to conduct a comparative study to follow gene loci and prominent chromosomal landmarks in both, chromosomes and"@en . "3226"^^ . . "1"^^ .