. . "5"^^ . "Bezvirov\u00E9 brambory - ozdraven\u00ED, diagn\u00F3za a molekul\u00E1rn\u00ED technologie indukovan\u00E9 rezistence"@cs . "This is the first part of proposals directed to the production of virus-free potatoes in our country. In this project the initial stages of the work will be solved, aiming at procedures of PVS elimination at in-vitro cultures, the development and evaluation of methods suitable of early virus detection and at increasing of the resistance by means of genetic-manipulation methods. Following tasks will be solved:1) the evaluation of virusstatic chemicals in combination with thermoterapy for more effective eradication of viruses and regeneration ability of explants, 2) the modification of immunochemical methods for early, sensitive and large-scale virus detection and for the evaluation of degree of resistance: the development of techniques based on molecular hybridisation and PCR for detection on transgenic materials as well as for the virus diagnosis, 3) the elaboration of the complex system of potato resistance against PVS emploing selected method of gene manipulation."@en . . "1316"^^ . "1316"^^ . "0"^^ . . . . . . . "Virus-free potatoes: health restoration, diagnosis and molecular technology of induced resistance"@en . . . "1"^^ . "V t\u00E9to prvn\u00ED \u010D\u00E1sti komplexn\u00EDho n\u00E1vrhu k zabezpe\u010Den\u00ED produkce zcela bezvirozn\u00EDch materi\u00E1l\u016F brambor budou \u0159e\u0161eny v\u00FDchoz\u00ED etapy pr\u00E1ce, zab\u00FDvaj\u00EDc\u00ED se postupu eliminace viru S (PVS) in vitro, v\u00FDvojem a ov\u011B\u0159ov\u00E1n\u00EDm metod vhodn\u00FDch pro do\u010Dasnou detekci viru a zvy\u0161ov\u00E1n\u00EDm \u00FArovn\u011B rezistence metodami genov\u00FDch manipulac\u00ED. Projekt zahrnuje: 1) testov\u00E1n\u00ED vlivu virostatick\u00FDch l\u00E1tek v kombinaci s termoterapi\u00ED na \u00FA\u010Dinn\u011Bj\u0161\u00ED inhibici viru a regera\u010Dn\u00ED schopnost explan\u00E1t\u016F 2)modifikace imunochemick\u00FDch postup\u016F diagn\u00F3zy pro v\u010Dasnou, citlivou a seriovou detekci viru a pro kvantifikaci zm\u011Bn v \u00FArovni rezistence: v\u00FDvoj molekul\u00E1rn\u011B - hybridiza\u010Dn\u00EDch technik a PCR jak pro detekci transgenn\u00EDch materi\u00E1l\u016F, tak viru 3)rozpracov\u00E1n\u00ED komplexn\u00EDho syst\u00E9mu odolnosti brambor k viru S pomoc\u00ED vybran\u00FDch metod genov\u00FDch manipulac\u00ED."@cs . "0"^^ . "5"^^ .