"Infection of wheat by fungus B. graminis is causing powdery mildew disease. Recently, non-race-specific powdery mildew resistance gene QPm.tut-4A was mapped on distal end of long arm of wheat chromosome 4A. Here we propose to construct two BAC DNA libraries specific for 4AL chromosomal arm. The huge genome and polyploid character hamper positional cloning in wheat. The proposed libraries would become keys for solving the obstruction and stepping stones for creation of physical map of the 4A chromosome. The physical map will facilitate cloning of important genes from the chromosome, including resistance genes to powdery mildew, leaf rust, Hessian fly, yellow rust, stem rust, wheat streak mosaic and Septoria tritici blotch. The main objective of the project is cloning of the powdery mildew resistance gene QPm.tut-4A. High density map of the QPm.tut-4A gene region will be created and BAC contig spanning the region identified. Subsequently, a pooled genomic BAC library will be prepared from the donor of resistance, T. militinae, and will be used to clone the QPm.tut-4A gene."@en . . "Konstrukce BAC knihoven specifick\u00FDch pro chromosom 4AL a pozi\u010Dn\u00ED klonov\u00E1n\u00ED genu pro rezistenci dosp\u011Bl\u00FDch rostlin k padl\u00ED travn\u00ED u p\u0161enice"@cs . . . "0"^^ . . "2"^^ . "3959"^^ . "2"^^ . . "1"^^ . . . "1"^^ . . "Construction of BAC DNA libraries specific for chromosome 4AL, and positional cloning of gene for adult plant resistance to powdery mildew in wheat"@en . "Triticum aestivum; BAC; chromoz\u00F3mov\u011B specifick\u00E9 knihovny, pozi\u010Dn\u00ED klonov\u00E1n\u00ED; padl\u00ED travn\u00ED"@en . . "2011-12-31+01:00"^^ . "C\u00EDlem navrhovan\u00E9ho projektu je konstrukce BAC DNA knihoven z dlouh\u00E9ho ramene p\u0161eni\u010Dn\u00E9ho chromosomu 4A pro p\u0159\u00EDpravu vysoko-hustotn\u00ED a fyzick\u00E9 mapy regionu genu QPm.tut-4A odpov\u011Bdn\u00E9ho za zv\u00FD\u0161enou rezistenci k padl\u00ED travn\u00ED a klonov\u00E1n\u00ED tohoto genu."@cs . . . "3959"^^ . . "2008-01-01+01:00"^^ .