"3634"^^ . "Processing of complex acoustical signals in the auditory thalamus and cortex and its changes in pathological conditions"@en . "7803"^^ . . . . . . "The aim of the project is to investigate the processing of complex acoustical signals such as vocalization in the neuronal circuits of the auditory cortex in the rat and guinea pig. Single- unit or multiple-unit activity will be recorded extracellularly with microelectrodes in anaesthetized or awake animals, and the interaction of adjacent units will be analyzed by the aim of crosscorrelation technique. The results will be systematically compared with the results obtained in previous studies performed under similar conditions in the inferior colliculus and medial geniculate body. As a part of the project, the delineation of several auditory cortical areas will be studied by using cytochrome oxidase and NADPH-diaphorase histochemistry and calbindin and parvalbumin immunocytochemistry. These techniques will also be employed in the assessment of changes in the neurochemistry of the central auditory syst\u00E9m after acoustic overstimulation and peripheral lesions and during aging. The electrophysiological, mo"@en . "C\u00EDlem pokus\u016F je v\u00FDzkum zpracov\u00E1n\u00ED komplexn\u00EDch akustick\u00FDch sign\u00E1l\u016F (nap\u0159. vokalizac\u00ED) v neuronov\u00FDch okruz\u00EDch sluchov\u00E9 k\u016Fry potkana a mor\u010Dete. Aktivita jednotliv\u00FDch neuron\u016F a skupin neuron\u016F bude sn\u00EDm\u00E1na extracelul\u00E1rn\u011B mikroelektrodou u narkotizovan\u00FDch nebobd\u011Bl\u00FDch zv\u00ED\u0159at a interakce sousedn\u00EDch neuron\u016F bude vyhodnocov\u00E1na kroskorela\u010Dn\u00ED technikou. V\u00FDsledky pokus\u016F budou systematicky porovn\u00E1v\u00E1ny s v\u00FDsledky na\u0161ich p\u0159edchoz\u00EDch pokus\u016F, proveden\u00FDch za podobn\u00FDch podm\u00EDnek v podkorov\u00FDch sluchov\u00FDch centrech - collicul us inferior a corpus geniculatum mediale. Sou\u010D\u00E1st\u00ED pokus\u016F je i stanoven\u00ED hranic n\u011Bkolika sluchov\u00FDch oblast\u00ED k\u016Fry mor\u010Dete a potkana pomoc\u00ED histochemie cytochromoxyd\u00E1zy a NADPH-diafor\u00E1zy a imunocytochemie kalbindinu a parvalbuminu. Tyto techniky budou tak\u00E9pou\u017Eity p\u0159i stanoven\u00ED morfologick\u00FDch a neurochemick\u00FDch zm\u011Bn v centr\u00E1ln\u00EDm sluchov\u00E9m syst\u00E9mu po ohlu\u0161en\u00ED, perifern\u00EDch l\u00E9z\u00EDch a v pr\u016Fb\u011Bhu st\u00E1rnut\u00ED. Elektrofyziologick\u00E9, histochemick\u00E9 a histologick\u00E9 studie budou organicky prov\u00E1z\u00E1ny s behavior\u00E1ln\u00EDmi pokusy,"@cs . . . "0"^^ . . . "0"^^ . . "1"^^ . . "Zpracov\u00E1n\u00ED komplexn\u00EDch akustick\u00FDch sign\u00E1l\u016F ve sluchov\u00E9m thalamu a k\u016F\u0159e a zm\u011Bny za patologick\u00FDch podm\u00EDnek"@cs . "20"^^ . "20"^^ .