. "C\u00EDlem navrhovan\u00E9ho projektu je pokra\u010Dovat ve struktur\u00E1ln\u00EDm a funk\u010Dn\u00EDm mapov\u00E1n\u00ED sluchov\u00FDch jader mor\u010Dete a laboratorn\u00EDho potkana, tentokr\u00E1te zam\u011B\u0159en\u00E9ho na studium cytoarchitektoniky, nervov\u00FDch spoj\u016F a neurochemick\u00E9ho profilu v prim\u00E1rn\u00ED a sekund\u00E1rn\u00ED oblasti sluchov\u00E9 k\u016Fry (SK). Pro vymezen\u00ED jednotliv\u00FDch sluchov\u00FDch oblast\u00ED a pro detailn\u00ED pozn\u00E1n\u00ED morfologie SK budou pou\u017Eity histochemick\u00E9 a imunohistochemick\u00E9 studie na detekci GABA, GAD a v\u00E1pn\u00EDk v\u00E1\u017E\u00EDc\u00EDch protein\u016F v nervov\u00FDch bu\u0148k\u00E1ch. Membr\u00E1nov\u00E9 a neurochemick\u00E9 vlastnosti jednotliv\u00FDch neuron\u016F budou studov\u00E1ny metodou patch-clamp na mozkov\u00FDch \u0159\u00EDzc\u00EDch. Extracelul\u00E1rn\u00ED z\u00E1znamy activity jednotliv\u00FDch neuron\u016F nebo neuronov\u00FDch soubor\u016F n\u00E1m umo\u017En\u00ED korelovat struktur\u00E1ln\u00ED a ultrastruktur\u00E1ln\u00ED parametry SK se zp\u016Fsobem zpracov\u00E1n\u00ED akustick\u00FDch podn\u011Bt\u016F (\u010Dist\u00FDch t\u00F3n\u016F, komplexn\u00EDch syntetick\u00FDch podn\u011Bt\u016F a zv\u00ED\u0159ec\u00EDch vokalizac\u00ED) ve SK. Behavior\u00E1ln\u00ED metodou budeme studovat mo\u017Enou asymetrii sluchov\u00E9 k\u016Fry p\u0159i detekci pauzy v \u0161umu po jednostrann\u00E9 ablaci SK. \u00DAloha"@cs . . "1"^^ . "4801"^^ . "Physiological and anatomical bases for acouctical signal processing in primary and secondary areas of the auditory cortex"@en . "10"^^ . . "10"^^ . . "0"^^ . . . "Neuvedeno."@en . "We propose to continue in our systematic studies of the strucure and function of the auditory system in guinea pig and rat with the aim to understand the cytoarchitecture, connections, and neurochemical profile of the primary and secondary areas of the auditory cortex (AC). The histochemical and immunocytochemical studies (GABA, GAD and calcium binding proteins detection will be performed in order to establish the boundaries and detailed morphology of AC areas as well as to study mechanisms of plasticity in the auditory cortex. Membrane and synaptic properties of individual neurons will be studied using patch-clamp technique in brain slices. Extracellular recording of single- or multiple-unit activity will enable us to correlate the structuraland ultrastructural AC parameters with the processing of acoustical signals such as tones, complex synthesized stimuli or species-specific vocalizations. Behavorial methods will be used to test a possible cortical asymetry in gap detection using"@en . . "Fyziologick\u00E9 a anatomick\u00E9 z\u00E1klady zpracov\u00E1n\u00ED akustick\u00FDch podn\u011Bt\u016F v prim\u00E1rn\u00EDch a sekund\u00E1rn\u00EDch oblastech sluchov\u00E9 k\u016Fry"@cs . . . . "4801"^^ . "1"^^ . .