"amphiphilic block copolymers; peptide segments; supramolecular structures; folding; molecular dynamics"@en . "2"^^ . "1883"^^ . . . "0"^^ . . "5"^^ . . . "Nadmolekul\u00E1rn\u00ED struktury amfifiln\u00EDch blokov\u00FDch kopolymer\u016F obsahuj\u00EDc\u00EDch peptidov\u00E9 segmenty"@cs . "5"^^ . . . "1883"^^ . . "1"^^ . "Hydrogel-like biodegradable copolymers featuring practical applications can be prepared combining poly(ethylene oxide) hydrophilic chains with hydrophobic oligopeptide segments (OS's containing several charged (either positively or negatively) sidechains into a-helical fragments. Solid-phase peptide synthesis will be employed to prepare suitably chosen (based on molecular dynamics (MD) calculations) OS's. IR, Raman, UV, CD, and NMR, both liquid- and solid-state, spectroscopies will be used to study folding and hydration patterns of OS's. The hydrophobic- and Coulombic-driven association of OS's, i.e., the polymerization of the corresponding a-amino acid N-carboxy anhydrides, will be used and supplemented with MD simulations of resulting mutations in amino acid' sequences."@en . . "Supramolecular Structures of Amphiphilic Block Copolymers Containing Oligopeptide Segments"@en . "Biodegradovateln\u00E9 kopolymery lze z\u00EDskat z hydrofiln\u00EDch \u0159et\u011Bzc\u016F poly(ethylen oxidu) a hydrofobn\u00EDch oligopeptidov\u00FDch segment\u016F (OS). Bude zkoum\u00E1n proces skl\u00E1d\u00E1n\u00ED OS (obsahuj\u00EDc\u00EDch krom\u011B hydrofiln\u00EDch i kladn\u011B, resp. z\u00E1porn\u011B nabit\u00E9 postrann\u00ED \u0159et\u011Bzce) do a-helix\u016F. OS budou p\u0159ipraveny synt\u00E9zou na pevn\u00E9m nosi\u010Di a po\u010D\u00EDta\u010Dov\u011B modelov\u00E1ny metodami molekulov\u00E9 dynamiky (MD, k jejich charakterizaci a studiu skl\u00E1d\u00E1n\u00ED bude pou\u017Eita spektroskopie I\u010C, Ramanova, UV, CD a NMR. Bude studov\u00E1na asociace OS ve vodn\u00FDch roztoc\u00EDch ur\u010Dov\u00E1n\u00EDm afinity, stechiometrie, specificity a kinetick\u00FDch parametr\u016F. Analogick\u00E9 OS budou p\u0159ipravov\u00E1ny technologicky jednodu\u0161\u0161\u00ED (a t\u00EDm zaj\u00EDmav\u011Bj\u0161\u00ED pro o\u010Dek\u00E1van\u00E9 praktick\u00E9 aplikace) polymerac\u00ED N-karboxyanhydrid\u016F p\u0159\u00EDslu\u0161n\u00FDch a-aminokyselin. Pomoc\u00ED MD bude simulov\u00E1no, jak zm\u011Bny v sekvenci OS vznikl\u00E9 pou\u017Eit\u00EDm statistick\u00E9 polymerace ovlivn\u00ED prostorovou strukturu a tvorbu komplex\u016F kopolymer\u016F. Z\u00EDskan\u00E9 poznatky bude mo\u017Eno vyu\u017E\u00EDt pro n\u00E1vrhy nov\u00FDch biokompatibiln\u00EDch materi\u00E1l\u016F."@cs .