. . "The goal of this project is the elucidation of the processes of assembly and budding of mouse mammary tumor virus (MMTV), the prototypical representative of the betaretrovirus genus. The study of virion formation will involve the development of an in vitro assembly system and analysis of morphogenesis in vivo in tissue culture. Individual protein domains within the major structural polyprotein Gag will be deleted, altered, or substituted with well characterized domains from other retroviruses, and the influence upon assembly and budding will be studied. All Gag domains unique to MMTV will be characterized and their specific contributions to infectious particle formation investigated. Cellular proteins that take part in intracellular transport and budding of preassembled viral capsids will be identified through a yeast two-hybrid screen of a mouse cDNA library."@en . "1"^^ . "894"^^ . . "1"^^ . "MMTV; mouse mammary tumor virus; betaretrovirus; retrovirus assembly; L. domain"@en . . . "2007-12-01+01:00"^^ . . "894"^^ . . . "2005-01-01+01:00"^^ . "C\u00EDlem projektu je ur\u010Den\u00ED jednotliv\u00FDch dom\u00E9n a interak\u010Dn\u00EDch motiv\u016F uvnit\u0159 strukturn\u00EDho polyproteinu Gag, kter\u00E9 jsou nezbytn\u00E9 pro proces skl\u00E1d\u00E1ni betaretrovirov\u00E9 \u010D\u00E1stice. Studovan\u00FDm retrovirem je typick\u00FD z\u00E1stupce rodu betaretrovir\u016F, virus MMTV (mouse mammary tumor virus), kter\u00FD zp\u016Fsobuje n\u00E1dorov\u00E9 onemocn\u011Bn\u00ED u my\u0161\u00ED. Studium formov\u00E1n\u00ED virov\u00E9 \u010D\u00E1stice bude zahrnovat testy jak in vitro, tak in vivo v tk\u00E1\u0148ov\u00FDch kultur\u00E1ch. Jednotliv\u00E9 proteinov\u00E9 dom\u00E9ny uvnit\u0159 hlavn\u00EDho strukturn\u00EDho polyproteinu Gag budou deletov\u00E1ny, mutov\u00E1ny nebo nahrazeny ji\u017E charakterizovan\u00FDmi dom\u00E9nami jin\u00FDch retrovir\u016F (HIV, MPMV) a bude sledov\u00E1n vliv na skl\u00E1d\u00E1n\u00ED a pu\u010Den\u00ED virionu. V\u0161echny dom\u00E9ny specifick\u00E9 pro virus MMTV budou d\u00E1le charakterizov\u00E1ny a jejich p\u0159\u00EDsp\u011Bvky pro tvorbu infek\u010Dn\u00EDho virionu analyzov\u00E1ny. Bun\u011B\u010Dn\u00E9 proteiny, kter\u00E9 se \u00FA\u010Dastn\u00ED intracelul\u00E1rn\u00EDho transportu virov\u00FDch \u010D\u00E1stic nebo jejich pu\u010Den\u00ED prost\u0159ednictv\u00EDm interakce s Gag polyproteinem, budou identifikov\u00E1ny pomoc\u00ED test\u016F v dvouhybridov\u00E9m syt\u00E9mu."@cs . . . . . "0"^^ . "1"^^ . "Studium procesu skl\u00E1d\u00E1n\u00ED a pu\u010Den\u00ED \u010D\u00E1stic viru my\u0161\u00EDho tumoru prsn\u00ED \u017El\u00E1zy MMTV"@cs . "Assembly and budding of mouse mammary tumor virus"@en . "0"^^ .