. . . . . "\"U vybran\u00FDch druh\u016F okrasn\u00FDch rostlin bude vyvinut postup zalo\u017Een\u00FD na molekul\u00E1rn\u011B genetick\u00FDch metod\u00E1ch, kter\u00FD umo\u017En\u00ED zablokv\u00E1n\u00ED n\u011Bkter\u00FDch krok\u016F biosynt\u00E9zy kv\u011Btn\u00EDch barviv. C\u00EDle bude dosa\u017Eeno transformac\u00ED Dendranthema grandiflora a Rhododendron carawbiense bakteriemi Agrobacterium tumefaciens nesouc\u00EDmi \"\"antisense\"\" nebo \"\"sense\"\" konstrukty genu CHS biosyntetick\u00E9 dr\u00E1hy antokyan\u016F. Z\u00EDskan\u00E9 barevn\u00E9 varianty budou molekul\u00E1rn\u011B charakterizov\u00E1ny pomoc\u00ED rychl\u00E9 a spolehliv\u00E9 metody, je\u017E bude vyv\u00EDjena soub\u011B\u017En\u011B s prvn\u00ED \u010D\u00E1st\u00ED projektu na velmi cenn\u00E9 sb\u00EDrce kultivar\u016F topolu \u010Dern\u00E9ho. K tomu budou z fragment\u016F genomu po p\u0159edchoz\u00EDm obohacen\u00ED na streptavidinov\u00FDch sloupc\u00EDch s komplement\u00E1rn\u00EDmi biotinylovan\u00FDmi oligonukleotidy izolov\u00E1ny a sekvenov\u00E1ny 3b a 4b mikrosatelity. Vybran\u00E9 mikrosatelitov\u00E9 markery budou pot\u00E9 kombinov\u00E1ny p\u0159i v\u00FDvoji standardizovan\u00E9 multiplexov\u00E9 metody, kter\u00E1 umo\u017En\u00ED \u00FAplnou charakterizaci v jedn\u00E9 amplifika\u010Dn\u00ED reakci. Popsan\u00FDp ostup bude aplikov\u00E1n ina kultivarov\u00E9 sb\u00EDrky jin\u00FDch druh\u016F.\""@cs . . . "2"^^ . "0"^^ . . "0"^^ . "flower pigment biosynthesis;dendranthema grandiflora;rhododendron catawbiense;sense and antisense constructs;black poplar;microsatellite markers;standardized multiplex procedure"@en . "Vyu\u017Eit\u00ED gen. manipulaci a molekul\u00E1rn\u00EDch marker\u016F pro produkci v\u00FDchoz\u00EDho \u0161lechtit. materi\u00E1lu a charakterizaci kultivar\u016F okrasn\u00FDch rostlin a d\u0159evin"@cs . . "The use of gene manipulations and molecular markers for production of starting breeding materials and characterization of cultivars of ornamental plan"@en . "2"^^ . "7475"^^ . "2750"^^ . . "1"^^ . "\"Procedure for the selective inhibition of some flower pigment biosynthesis steps in selecte ornamental plants will be developed with the use of molecular biology methods. Agrobacterium tumefaciens transformation of Dendranthema grandiflora and Thododendron catawbiense is proposed, with \"\"sense\"\" and \"\"antisense\"\" construts from anthocyanin synthesis genes CHS, CHI or DFR. Flower pigment variant plants obtained will be characterized by quick and reliable DNA-fingerprinting method that will be developed in parallel research, on the collection of cultivars of black poplar at first. Microsatellites will be identified and sequenced in genome fragments enriched in 3b a 4b repeated sequences on streptavidine columns with biotin-bound complementary oligos. Useful microsatelite markers will be selected and combined to develop standardized multiplex procedure for the complete identification in one amplification reaction. The same approach will be used for collections of other plant species.\""@en .