"6638"^^ . "0"^^ . . "2"^^ . "C\u00EDlem projektu bude vyvinout immunodiagnostika pro anal\u00FDzu a \u010Di\u0161t\u011Bn\u00ED l\u00E1tek hormon\u00E1ln\u00ED povahy, a to zejm\u00E9na cytokinin\u016F, auxin\u016F, steroid\u016F a kyseliny abscisov\u00E9. Projekt se bude zab\u00FDvat v\u00FDvojem n\u00E1sleduj\u00EDc\u00EDch komponent: (1) synt\u00E9zou antigen\u016F, (2) p\u0159\u00EDpravou specifick\u00FDch polyklon\u00E1ln\u00EDch amonoklon\u00E1ln\u00EDch protil\u00E1tek, (3) synt\u00E9zou konjug\u00E1t\u016F hormon\u016F s enzymy (alkalickou fosfatasou \u010Di peroxidasou), (4) optimalizac\u00ED ELISA test\u016F, v\u010Detn\u011B studi\u00ED specifity (definice zk\u0159\u00ED\u017Een\u00E9 reaktivity se struktur\u00E1ln\u011B relevantn\u00EDmi l\u00E1tkami), (5) p\u0159\u00EDpravou kitu, (6) \u010Di\u0161t\u011Bn\u00EDm polyklon\u00E1ln\u00EDch protil\u00E1tek afinitn\u00ED chromatografi\u00ED (nezbytn\u00E9 pro imunoafinitn\u00ED chromatografii), (7) v\u00FDvojem imunokolon a jejich adjustace a charakterizace, (8) ov\u011B\u0159en\u00EDm pou\u017Eitelnosti ELISA kit\u016F i imunokolon na p\u0159irozen\u00FDch vzorc\u00EDch. Zav\u00E1d\u011Bny budou i perspektivn\u00ED metody velkokapacitn\u00ED produkce monoklon\u00E1ln\u00EDch protil\u00E1tek in vitro (bioreaktory). Po ukon\u010Den\u00ED v\u00FDvoje budou z\u00EDskan\u00E9 metodiky a postupy p\u0159ed\u00E1ny soukrom\u00FDm firm\u00E1m v \u010CR formou licen\u010Dn\u00EDch smluv."@cs . "16"^^ . . . . . "immunodiagnostics;polyclonal and monoclonal antibodies;ELISA kits;immunoaffinity chromatography;phytohormones;cytokinins;auxins;steroids;abscisic acid"@en . . "16"^^ . "Immunodiagnostics of natural hormonal substances"@en . . . "Imunodiagnostika p\u0159\u00EDrodn\u00EDch l\u00E1tek hormon\u00E1ln\u00ED povahy"@cs . . "1"^^ . . . "Aim of the project is to develop immunodiagnostics for analyses and purification of hormonal substances, preferentially cytokinins, auxins, steroids and abscisic acid. Project will be oriented to development of the following components: (1) syntheses of antigens, (2) syntheses of hormone conjugates with enzymes (alkaline phosphatase or peroxidase), (4) optimalization of ELISA assays and cross-reactivity studies with appropariate structural analogues, (5) preparation of kits, (6) purification of polyclonal antibodies by affinity chromatography (necessary for immunoaffinity chromatography), (7) development of immunocolumns and their characterization, (8) application of ELISA kits and immunocolumns on natural samples. We would also like to introduce high capacity production of monoclonal antibodies by in vitro technologies (bioreactors). After finishing of the project the obtained results and technologie will be transfered to private firms in the Czech Republic via license agreements."@en . "1670"^^ .