"2009-12-31+01:00"^^ . "3405"^^ . . "We will study an uptake and accumulation of selenium by green algae (Chlamydomonas, Scenedesmus, Chlorella). Changes in ratio of low-molecular weight forms of selenium and selenoproteins at different concentrations of selenium supplied and activity of selenoproteins GHX2, TR1 in different cell cycle phases will be followed. Localization of selenium in cells will be studied at ultra-thin sections by electronmicroscopy. By testing of different growth conditions(light intensity, spectral composition of light, temperature, CO2, nutrition), the best conditions for maximal production of selenium compounds in synchronous algal cultures will be found out. The part of the project will be a selection of strains with high selenium uptake ability. Results of laboratory experiments will be tested in production photobioreactors."@en . "selenium; selenoproteins; cell cycle; green algae"@en . . "16"^^ . . "16"^^ . . "Study of accumulation of selenium and its forms in the cell cycle of green algae."@en . "1"^^ . . "Budeme studovat p\u0159\u00EDjem a akumulaci selenu do bun\u011Bk zelen\u00FDch \u0159as (Chlamydomonas, Scenedesmus, Chlorella). Budou sledov\u00E1ny zm\u011Bny v pom\u011Bru n\u00EDzkomolekul\u00E1rn\u00EDch forem selenu a selenoprotein\u016F p\u0159i r\u016Fzn\u00FDch koncentrac\u00EDch dod\u00E1van\u00E9ho selenu a aktivita selenoprotein\u016F GHX2, TR1 v r\u016Fzn\u00FDch f\u00E1z\u00EDch bun\u011B\u010Dn\u00E9ho cyklu. Bun\u011B\u010Dn\u00E1 lokalizace selenu bude sledov\u00E1na na ultra tenk\u00FDch \u0159ezech pomoc\u00ED elektronov\u00E9 mikroskopie. Testov\u00E1n\u00EDm r\u016Fstov\u00FDch podm\u00EDnek (intenzita a spektr\u00E1ln\u00ED slo\u017Een\u00ED sv\u011Btla, teplota, CO2, v\u00FD\u017Eiva) a pou\u017Eit\u00EDm inhibi\u010Dn\u00EDch faktor\u016F (vysok\u00E1 teplota, UV z\u00E1\u0159en\u00ED, inhibitory) budou zji\u0161t\u011Bny nejvhodn\u011Bj\u0161\u00ED podm\u00EDnky pro maxim\u00E1ln\u00ED produkci selenov\u00FDch slou\u010Denin v synchronizovan\u00FDch \u0159asov\u00FDch kultur\u00E1ch. Sou\u010D\u00E1st\u00ED projektu bude tak\u00E9 selekce kmen\u016F \u0159as s vysokou schopnost\u00ED p\u0159\u00EDjmu selenu. V\u00FDsledky z laboratorn\u00EDch pokus\u016F budou testov\u00E1ny v podm\u00EDnk\u00E1ch produk\u010Dn\u00EDch fotobioreaktor\u016F."@cs . . "2007-01-01+01:00"^^ . "2"^^ . . "0"^^ . . . . "3405"^^ . "V\u00FDzkum akumulace selenu a jeho forem v pr\u016Fb\u011Bhu bun\u011B\u010Dn\u00E9ho cyklu zelen\u00FDch \u0159as"@cs . . .