"12"^^ . "5095"^^ . . "The main goal of this project is to understand the structural basis of 14-3-3 protein\u2013dependent regulation of function of RGS3 protein. 14-3-3 proteins are regulatory molecules that bind to other proteins in a phosphorylation dependent manner. RGS proteins function both as GTPase activating proteins for G proteins and regulators of G protein-effector interactions. RGS3 protein is one of the RGS proteins known to be 14-3-3 binding partners. The role of 14-3-3 protein in the regulation of RGS3 seems to be an inhibition of its GTPase activating function on G protein mediated signals. However structural details concerning interaction between 14-3-3 and RGS3 are unknown. We propose to perform (1) biophysical characterization of 14-3-3/RGS3 complex; (2) to study how 14-3-3 protein inhibits the interaction between RGS3 protein and Galpha subunit of G protein using fluorescence spectroscopy; (3) to perform structural analysis of the 14-3-3/RGS3 complex using protein X-ray crystallography."@en . . . . "12"^^ . "2008-01-01+01:00"^^ . "0"^^ . . "Study of the interaction between 14-3-3 protein and regulator of G protein signaling RGS3."@en . "1"^^ . . "Hlavn\u00EDm c\u00EDlem tohoto projektu je studium strukturn\u00ED podstaty regulace funkce RGS3 proteinu. 14-3-3 proteiny jsou regula\u010Dn\u00ED molekuly, kter\u00E9 specificky v\u00E1\u017E\u00ED fosforylovan\u00E9 proteiny. RGS proteiny maj\u00ED jednak funkci protein\u016F aktivuj\u00EDc\u00EDch GTPasovou aktivitu G protein\u016F a d\u00E1le reguluj\u00ED interakce mezi G proteiny a jejich efektory. RGS3 protein je jedn\u00EDm z RGS protein\u016F u kter\u00FDch bylo zji\u0161t\u011Bno, \u017Ee jsou vazebn\u00FDmi partnery 14-3-3 protein\u016F. Zd\u00E1 se, \u017Ee 14-3-3 proteiny funguj\u00ED jako inhibitory RGS protein\u016F \u2013 redukuj\u00ED jejich schopnost aktivovat GTPasovou aktivitu G protein\u016F. Molekul\u00E1rn\u00ED mechanismus funkce 14-3-3 proteinu v procesu regulace RGS3 proteinu je nezn\u00E1m\u00FD. Proto navrhujeme (1) prov\u00E9st biofyzik\u00E1ln\u00ED charakterizaci 14-3-3/RGS3 komplexu; (2) studovat jak\u00FDm zp\u016Fsobem 14-3-3 protein inhibuje interakci mezi RGS3 proteinem a Galfa podjednotkou G proteinu pomoc\u00ED fluorescen\u010Dn\u00ED spektroskopie; (3) prov\u00E9st strukturn\u00ED anal\u00FDzu komplexu 14-3-3 protein/RGS3 protein pomoc\u00ED proteinov\u00E9 rentgenov\u00E9 krystalografie."@cs . . . . "2012-12-31+01:00"^^ . "RGS3 protein; 14-3-3 protein; fluorescence; interaction; X-ray crystallography; phosphorylation"@en . "2"^^ . . . "5095"^^ . . "Studium interakce 14-3-3 proteinu s regul\u00E1torem G proteinov\u00E9 signalizace RGS3."@cs .