. . . . . "16"^^ . "crystallization of polymers; protein crystallography, X-ray structure analysis; protein hydration; self-organization of molecules; macromolecular systems; crystallization buffer; polymer precipitant; intermolecular adhesion"@en . "2786"^^ . "Persistent problem of the protein crystallography is low efficiency of the crystallization process. Polymer precipitants used in our structure determinations of 30 protein complexes showed high efficacy. They often proved to bind partly at specific sites on the protein surface and partly they remain solvated in buffer filling 40-80% of the crystal volume. Preliminary analysis shows that the efficacy of these precipitants is high and that its function consists in selective blocking the potential adhesion sites on the surface of the target macromolecule. Protein surface possess a number of potential adhesion sites, however the crystal of diffraction quality appears when only a single adhesion site is active during crystal growth. The project analyzes experimentally and theoretically this phenomenon, i.e. quality of adhesion sites, types of blocking ligands and conditions for their use. Parallel analysis of all components and parameters of crystallization solution is necessary."@en . "2011-12-31+01:00"^^ . "0"^^ . "2007-01-01+01:00"^^ . "Self-organization, crystallization and structure determination of protein based macromolecular systems"@en . . . . "0"^^ . . "2786"^^ . . "1"^^ . . . "16"^^ . "Probl\u00E9mem p\u0159i stanoven\u00ED struktur protein\u016F je n\u00EDzk\u00E1 \u00FAsp\u011B\u0161nost p\u0159i p\u0159\u00EDprav\u011B krystal\u016F difrak\u010Dn\u00ED kvality. Zjistili jsme, \u017Ee polymern\u00ED precipitanty, kter\u00E9 jsme pou\u017Eili p\u0159i \u0159e\u0161en\u00ED struktur cca 30 komplex\u016F protein\u016F,\u010Dasto z\u016Fst\u00E1vaj\u00ED svoj\u00ED \u010D\u00E1st\u00ED adsorbovan\u00E9 na povrchu protein\u016F, p\u0159i\u010Dem\u017E jejich v\u011Bt\u0161\u00ED \u010D\u00E1st z\u016Fst\u00E1v\u00E1 rozpu\u0161t\u011Bn\u00E1 v pufru vypl\u0148uj\u00EDc\u00EDm 40-80 % prostoru krystalu. P\u0159edb\u011B\u017En\u00E1 anal\u00FDza ukazuje, \u017Ee \u00FAsp\u011B\u0161nost t\u011Bchto precipitant\u016F je vysok\u00E1 a \u017Ee spo\u010D\u00EDv\u00E1 v r\u016Fzn\u00E9m stupni blokov\u00E1n\u00ED specifick\u00FDch sorp\u010Dn\u00EDch m\u00EDst na povrchu c\u00EDlov\u00E9 makromolekuly. Proteiny maj\u00ED na sv\u00E9m povrchu velk\u00FD po\u010Det potenci\u00E1ln\u00EDch sorp\u010Dn\u00EDch m\u00EDst, ale krystal difrak\u010Dn\u00ED kvality vznikne jen pokud je preferov\u00E1na pouze jedin\u00E1 mo\u017Enost adheze. Tento projekt je zam\u011B\u0159en na experiment\u00E1ln\u00ED a teoretickou anal\u00FDzu tohoto jevu, tj. jak vypadaj\u00ED adhezn\u00ED m\u00EDsta na povrchu proteinu dostupn\u00E1 pro blokuj\u00EDc\u00ED ligandy, jak\u00E9 ligandy pou\u017E\u00EDt a za jak\u00FDch podm\u00EDnek jsou \u00FAsp\u011B\u0161n\u00E9. P\u0159itom je samoz\u0159ejm\u011B t\u0159eba sou\u010Dasn\u011B analyzovat roli v\u0161ech slo\u017Eek krystaliza\u010Dn\u00EDho roztoku."@cs . "Vnit\u0159n\u00ED organizace makromolekul\u00E1rn\u00EDch syst\u00E9m\u016F, krystalizace a stanoven\u00ED struktury makromolekul\u00E1rn\u00EDch syst\u00E9m\u016F obsahuj\u00EDc\u00EDch proteiny"@cs .