"cardiomyocyte; anoxia; calcium; chronic hypoxia; cardioprotection; right and left ventricle"@en . . . "1512"^^ . "1"^^ . "Anoxic tolerance and Ca2+ homeostasis of right and left ventricular myocytes isolated from chronically hypoxic hearts"@en . "0"^^ . "1"^^ . "1"^^ . . "2010-12-31+01:00"^^ . . . . . . . "1512"^^ . "Myocardial ischemia belongs to the most frequent diseases of modern times. Adaptation to chronic intermittent hypoxia (IH) is known to protect the heart against ischemic injury for a prolonged period of time. Several pathways have been proposed to play a role in IH-induced protection but the exact mechanism is unknown. This project will focus on chamber- and time-dependent protective effects of chronic IH and sustained hypoxia (SH) examined on isolated myocytes. It is expected to clarify, whether periodic reoxygenation rather hypoxia per se is responsible for the improved ischemic tolerance of IH hearts. Viability of right and left ventricular myocytes subjected to acute anoxia/reoxygenation, and LDH release will be determined. Electrically- and caffeine-induced calcium transients will provide information on calcium homeostasis. The subcellular distribution of protein kinase C isoenzymes will be examined to delineate protective pathways involved in this form of cardioprotection."@en . . "2008-01-01+01:00"^^ . "Rezistence k anoxii a Ca2+ homeost\u00E1za kardiomyocyt\u016F z prav\u00E9 a lev\u00E9 komory chronicky hypoxick\u00FDch potkan\u016F"@cs . . . "0"^^ . "Akutn\u00ED ischemie myokardu pat\u0159\u00ED k hlavn\u00EDm p\u0159\u00ED\u010Din\u00E1m \u00FAmrt\u00ED v dne\u0161n\u00EDm sv\u011Bt\u011B. Adaptace na chronickou intermitentn\u00ED hypoxii (IH) chr\u00E1n\u00ED srdce p\u0159ed ischemick\u00FDm po\u0161kozen\u00EDm po mnohem del\u0161\u00ED dobu ne\u017E tzv. preconditioning. V protektivn\u00EDm p\u016Fsoben\u00ED IH se uplat\u0148uje \u0159ada faktor\u016F, ale detailn\u00ED molekul\u00E1rn\u00ED mechanismus nebyl dosud objasn\u011Bn. Projekt je zam\u011B\u0159en na sledov\u00E1n\u00ED \u010Dasov\u00E9ho pr\u016Fb\u011Bhu a region\u00E1ln\u00EDch rozd\u00EDl\u016F v protektivn\u00EDm p\u016Fsoben\u00ED chronick\u00E9 IH a kontinu\u00E1ln\u00ED hypoxie (SH) na \u00FArovni izolovan\u00FDch kardiomyocyt\u016F. Pokus\u00ED se objasnit, zda za zv\u00FD\u0161enou ischemickou odolnost chronicky hypoxick\u00FDch srdc\u00ED je zodpov\u011Bdn\u00E1 periodick\u00E1 reoxygenace (spojen\u00E1 s IH) \u010Di hypoxie samotn\u00E1. Budeme sledovat viabilitu a funk\u010Dn\u00ED stav myocyt\u016F, izolovan\u00FDch z prav\u00E9 a lev\u00E9 komory, ve vztahu k v\u00E1pn\u00EDkov\u00E9 komeost\u00E1ze a subcelul\u00E1rn\u00ED distribuci isoenzym\u016F protein kin\u00E1zy C. Pl\u00E1novan\u00E9 experimenty by m\u011Bly p\u0159in\u00E9st nov\u00E9 poznatky o \u00FAloze oxidativn\u00EDho stresu v indukci adaptivn\u00EDch odpov\u011Bd\u00ED vedouc\u00EDch ke zv\u00FD\u0161en\u00E9 odolnosti chronicky hypoxick\u00FDch srdc\u00ED."@cs .