. "3428"^^ . . "10"^^ . "10"^^ . . . "Using the experimental and theoretical methods for structure analysis of HIV protease complexes with selected inhibitors"@en . . "0"^^ . "9361"^^ . "C\u00EDlem projektu je zav\u00E9st rutinn\u011B metodu \u0159e\u0161en\u00ED struktury protein\u016F, stanovit struktury komplex\u016F HIV prote\u00E1zy s vybran\u00FDmi inhibitory, ov\u011B\u0159it tak p\u0159edpokl\u00E1dan\u00FD zp\u016Fsob komplexace, analyzovat p\u0159\u00ED\u010Diny rozd\u00EDl\u016F v inhibici nativn\u00ED a mutovan\u00E9 prote\u00E1zy a pokusit se o zobecn\u011Bn\u00ED dosud shrom\u00E1\u017Ed\u011Bn\u00FDch dat k n\u00E1vrhu \u00FA\u010Dinn\u00E9ho l\u00E9\u010Diva proti AIDS. V r\u00E1mci prob\u00EDhaj\u00EDc\u00EDch projekt\u016F v \u00DAMG a na \u00DAOCHB AV \u010CR jsou \u00FAsp\u011B\u0161n\u011B vyv\u00EDjeny nezbytn\u00E9 metody zaji\u0161\u0165uj\u00EDc\u00ED 1) bezpe\u010Dnou produkci dostat\u010Dn\u00E9ho mno\u017Estv\u00ED nativn\u00ED i mutovan\u00E9 prote\u00E1zy isolovan\u00E9 z pacient\u016F, u nich\u017E do\u0161lo k resistenci na st\u00E1vaj\u00EDc\u00ED l\u00E9ky (Saquinavir) a jednak pro 2) rychl\u00E9 vyhled\u00E1v\u00E1n\u00ED \u00FA\u010Dinn\u00FDch inhibitor\u016F pomoc\u00ED techniky syntetick\u00FDch knihoven. D\u016Fvodem tohoto v\u00FDzkumu je, \u017Ee st\u00E1vaj\u00EDc\u00ED l\u00E9\u010Diva proti AIDS zalo\u017Een\u00E1 na inhibici HIV prote\u00E1z nejsou resistentn\u00ED proti schopnosti viru produkovat mutanty prote\u00E1zy, kter\u00E9 nejsou l\u00E9\u010Divem inhibov\u00E1ny a st\u00E1le umo\u017E\u0148uj\u00ED zr\u00E1n\u00ED viru."@cs . "0"^^ . "The aim is the determine structure of HIV protease complexes with selected inhibitors, to verify the supposed way of complexation, to analyse the cause of the inhibition differences observed with wild and mutated protease and to generalize the structure data for a design of a drug effective againt AIDS. Methods for safe production of wild and mutant protease isolated from patients with developed resistence to Saquinavir and for efficient scan for inhibitors using synthetic library techniques have been developed in frame of running projects in IOCB and IMG AS CR. Handicap of the present medicaments used against AIDS based on HIV protease inhibition is an ability of virus to produce the mutants which enable the virus live cycle, however are already not inhibited by the drug used. Structure determination using x-ray diffraction is necessary for a design of the permanently effective inhibitors."@en . "Inhibition of HIV protease; structure determination of HIV protease mutants; tetrapeptide inhibitors; X-ray structure analysis; molecular modelling;"@en . . . . "3"^^ . . "Vyu\u017Eit\u00ED experiment\u00E1ln\u00EDch a teoretick\u00FDch metod p\u0159i stanoven\u00ED struktury komplex\u016F HIV prote\u00E1zy s vybran\u00FDmi inhibitory"@cs . . .