"0"^^ . . . "1"^^ . "By CD spectroscopy and PAGE we propose to study the problems with synthetic cytosine-rich NA fragments: Characterization of a thermostable conformer that oligo(dC) forms at neutral pH, and its role in the formation of cytosine i-quadruplex. The anomalous conformation of d(CnGn) oligonucleotides.Duplex,G-quadruplex and i-quadruplex formation in the d(CnGn) and d(GnCn) fragments of DNA, their competition and intramolecular coexistence. Bimolecular duplex-foldback equilibria in the (G+C) - rich oligonucleotides. Unusual conformations of the regulerly alternating d(IC)n, d(TC)n and d(AC)n motifs. Role of alkyl, alkenyl and alkynyl substituents in position 5 of cytosine in the conformational properties of the oligonucleotides. Comparison of crystal and solution conformations of G+C)-rich DNA fragments.These studies will help to understand the role of cytosine clustering, protonation, methylation and regular alternation in the conformational polymorphism of cytosine-rich genomic regions."@en . "0"^^ . . "3"^^ . . . . "1425"^^ . "3"^^ . . . . "\u00DAloha cytosinu v konforma\u010Dn\u00ED polymorfii DNA"@cs . "Pomoc\u00ED CD spektroskopie, PAGE a dal\u0161\u00EDch metod budou \u0159e\u0161eny n\u00E1sleduj\u00EDc\u00ED probl\u00E9my:I)Charakterizace termostabiln\u00EDho konformeru, kter\u00FD tvo\u0159\u00ED oligo(dC) p\u0159i neutr\u00E1ln\u00EDm pH, jeho \u00FAloha p\u0159i tvorb\u011B interkalovan\u00E9ho cytosinov\u00E9ho kvadruplexu. II) Anom\u00E1ln\u00ED konformace oligonukleotid\u016F d(CnGn).III) Tvorba duplexu, quaninov\u00E9ho a cytosinov\u00E9ho kvadruplexu u d(CnGn) a d(GnCn), jejich kompetice a intramolekul\u00E1rn\u00ED koexistence. IV) Konforma\u010Dn\u00ED rovnov\u00E1hy bimolekul\u00E1rn\u00EDho duplexu a monomolekul\u00E1rn\u00EDho vl\u00E1senkov\u00E9ho uspo\u0159\u00E1d\u00E1n\u00ED u oligonukleotid\u016F bohat\u00FDch na (G+C). V)Neobvykl\u00E9 konformace motiv\u016F d(IC)n, d(TC)n a d(AC)n. VI) Ovlivn\u011Bn\u00ED konforma\u010Dn\u00EDch vlastnost\u00ED oligonukleotid\u016F alkylov\u00FDmi, alkenylov\u00FDmi a alkynylov\u00FDmi substituenty v poloze 5 cytosinu. VII) Srovn\u00E1n\u00ED konformac\u00ED fragment\u016F DNA bohat\u00FDch na (G+C) v krystalech roztoku. V\u00FDsledky pomohou pochopit \u00FAlohu shlukov\u00E1n\u00ED cytosin\u016F, jejich protonizace, metylace a pravideln\u00E9ho st\u0159\u00EDd\u00E1n\u00ED s jin\u00FDmi b\u00E1zemi v konforma\u010Dn\u00ED polymorfii oblast\u00ED genom\u016F bohat\u00FDch na cytosin."@cs . "3750"^^ . "Role of cytosine in the conformational polymorphism of DNA"@en . . .