"Navrhujeme vyvinout rychl\u00FD, jednoduch\u00FD, selektivn\u00ED a modifikovateln\u00FD elektrochemick\u00FD biodetektor (biosenzor) pro po\u0161kozen\u00ED DNA (jak in vitro, tak v bu\u0148k\u00E1ch) a pro l\u00E1tky po\u0161kozuj\u00EDc\u00ED DNA. Z\u00E1klad tohoto v\u00FDzkumu bude tvo\u0159it metoda vyvinut\u00E1 v na\u0161\u00ED laborato\u0159i umo\u017E\u0148uj\u00EDc\u00ED detekci zlom\u016F v DNA pomoc\u00ED rtu\u0165ov\u00FDch elektrod modifikovan\u00FDch nad\u0161roubovicovou DNA. V r\u00E1mci tohoto projektu bude na\u0161e pr\u00E1ce zam\u011B\u0159ena na n\u00E1sleduj\u00EDc\u00ED hlavn\u00ED t\u00E9mata: (1) Dal\u0161\u00ED zdokonalen\u00ED detekce zlom\u016F v DNA s vyu\u017Eit\u00EDm p\u0159edev\u0161\u00EDm stu\u0165ov\u00FDch filmov\u00FDch elektrod (MFE), a optimalizace anal\u00FDzy DNA na MFE. (2) Modifikace d\u0159\u00EDve vyvinut\u00E9 metody v techniku umo\u017E\u0148uj\u00EDc\u00ED detekci zlom\u016F v eukaryotick\u00E9 chromozom\u00E1ln\u00ED DNA. (3) Detekce po\u0161kozen\u00ED baz\u00ED v DNA s vyu\u017Eit\u00EDm enzym\u016F z\u00FA\u010Dastn\u011Bn\u00FDch v opravn\u00FDch procesech DNA. Enzymy budou vyu\u017Eity jak pro \u0161t\u011Bpen\u00ED po\u0161kozen\u00E9 DNA v roztoku, tak na povrchu elektrody. (4) Vyu\u017Eit\u00ED v\u00FDsledk\u016F z\u00EDskan\u00FDch v (1-3) pro anal\u00FDzu DNA po\u0161kozenou in vivo."@cs . . . . "Development of electrochemical biodetectors for DNA damage"@en . . . . . "1"^^ . "biosensor; biodetector; electrochemical detection; DNA damage; interactions; strand breaks; radicals; drugs; mutagens; carcinogens; environmental pollutants;"@en . . . "1758"^^ . "804"^^ . "14"^^ . "14"^^ . . . "0"^^ . "It is proposed to develop a fast response, simple, selective and versatile electrochemical biodetector (biosensor) for DNA damage (both in vitro and in cells) and DNA damaging agents. A procedure developed in our laboratory which allows to detect DNA strand breaks using mercury electrodes modified with supercoiled DNA will represent the ground of this research. Within this projects, our efferts will be focused mainly the following topics: (1) Improvement of detection of DNA strand breaks using mainlymercury film electrodes (MFE, and optimization of DNA analysis with the MFE. (2) Modification of the previously developed protocol into a technique allowing sensitive detection of DNA cleavage using eukaryotic chromosomal DNAs. (3) Detection of damage toDNA bases using DNA repair enzymes. The enzymes will be applied both in solution and at the electrode surface. (4) Using the results obtained in (1-3), analysis of DNA damaged in vivo."@en . "0"^^ . "V\u00FDvoj elektrochemick\u00FDch biodetektor\u016F pro po\u0161kozen\u00ED DNA"@cs .