. "1548"^^ . . "2"^^ . . . "Ion-selective electrodes with improved detection limit for analysis in biological fluids"@en . . "Iontov\u011B selektivn\u00ED elektrody se sn\u00ED\u017Een\u00FDm detek\u010Dn\u00EDm limitem pro anal\u00FDzu v biologick\u00FDch tekutin\u00E1ch"@cs . . . "ion-selective electrode; improved detection limit; biological fluids"@en . "10"^^ . . "1548"^^ . "10"^^ . "2009-12-31+01:00"^^ . "0"^^ . "The purpose of the proposed research is to extend the application of ion-selective electrodes (ISE) with improved detection limit for a determination of clinically relevant ions (Na+, K+, NH4+, Mg2+, Ca2+) in diluted blood plasma and urine samples. This approach should decrease the cost of clinical tests, and increase the lifetime of the sensing membranes by preventing their biofouling. Following issues will be addressed: (1) potentiometry of ISE with eliminating effects of zero-current ion fluxes, (2) ion amperometry of the polymeric ion-selective membranes, (3) impedance spectroscopy and computer simulation of ion concentration profiles in the membrane, (4) use of ISEs for a determination of inorganic ions in biological fluids. We expect that the results of this study will allow us to clarify the origin of the ISE potential, to assess the efficiency of various procedures leading to a lowering of the detection limit, and to develop a novel methodology for biomedical applications."@en . "2007-01-01+01:00"^^ . "\u00DA\u010Delem p\u0159edkl\u00E1dan\u00E9ho projektu bude roz\u0161\u00ED\u0159en\u00ED aplika\u010Dn\u00EDch mo\u017Enost\u00ED iontov\u011B selektivn\u00EDch elektrod (ISE) se sn\u00ED\u017Een\u00FDm detek\u010Dn\u00EDm limitem pro stanoven\u00ED klinicky v\u00FDznamn\u00FDch iont\u016F (Na+, K+, NH4+, Mg2+, Ca2+) ve z\u0159ed\u011Bn\u00E9 krevn\u00ED plasm\u011B a mo\u010Di. Navr\u017Een\u00FD p\u0159\u00EDstup povede ke sn\u00ED\u017Een\u00ED n\u00E1klad\u016F na klinick\u00E9 testy a k prodlou\u017Een\u00ED \u017Eivotnosti iontov\u011B selektivn\u00EDch membr\u00E1n zamezen\u00EDm jejich degradace p\u0159i styku s biologick\u00FDm materi\u00E1lem. Pozornost bude v\u011Bnov\u00E1na n\u00E1sleduj\u00EDc\u00EDm t\u00E9mat\u016Fm: (1) potenciometrie ISE s eliminac\u00ED vlivu iontov\u00FDch tok\u016F za podm\u00EDnek nulov\u00E9ho proudu, (2) iontov\u00E1 amperometrie iontov\u011B selektivn\u00EDch membr\u00E1n, (3) impedan\u010Dn\u00ED spektroskopie a po\u010D\u00EDta\u010Dov\u00E9 simulace profil\u016F iontov\u00FDch koncentrac\u00ED uvnit\u0159 membrany, (4) pou\u017Eit\u00ED ISE pro stanoven\u00ED anorganick\u00FDch inont\u016F v biologick\u00FDch tekutin\u00E1ch. P\u0159edpokl\u00E1d\u00E1me, \u017Ee v\u00FDsledky studie d\u00E1le povedou k objasn\u011Bn\u00ED p\u016Fvodu potenci\u00E1lu ISE, k posouzen\u00ED \u00FA\u010Dinnosti r\u016Fzn\u00FDch p\u0159\u00EDstup\u016F vedouc\u00EDch ke sn\u00ED\u017Een\u00ED detek\u010Dn\u00EDho limitu a k v\u00FDvoji nov\u00E9 metodologie pro biomedicin\u00E1ln\u00ED aplikace."@cs . . "1"^^ . . .