"Vyu\u017Eit\u00ED kombinace laserov\u00FDch mikrosvazkov\u00FDch a cytometrick\u00FDch technik ke studiu struktury a dynamiky lidsk\u00E9ho genomu"@cs . "47"^^ . "The aim of the project is to use the methods and experimental systems of optical tweezers, optical scalpel, and automated cytometer, that were developed using the finantial support of the previous scientific projects, for the study of the mechanical properties and arrangement of the interphase chromatin in the nuclei of living and fixed human cells. Theoretical and experimental experiences of the interdisciplinary team of applicants will be used for: - fluorescence labelling of chromatin in living cells, - laser induced fusion of labelled cells, - topologic analyses of the dye distribution inside living and fixed cells, - insertion of dyed nanoparticle into the living cell nucleus, optical confinement of this probe and detection of the region which is not accesible for the probe, - study of the mechanical properties of the chromatin using optical trapping of suitable probes. The knowledge obtained by these techniques will be used for development of new diagnostic methods."@en . . "5916"^^ . "47"^^ . . "optical-tweezers; optical-scalpel; high-resolution-cytometer; confocal- microscopy; chromatin-structure; nuclear-structure; chromosome-domains; chromosome-aberrations; human-genome; genomics; diagnostics-in-oncology; cell-fusion; image-analyses"@en . "3786"^^ . . "0"^^ . . "1"^^ . "Podstatou projektu je vyu\u017E\u00EDt metod a experiment\u00E1ln\u00EDch sestav optick\u00E9 pinzety, optick\u00E9ho skalpelu a automatizovan\u00E9ho cytometru, kter\u00E9 byly vyvinuty za finan\u010Dn\u00ED podpory p\u0159edch\u00E1zej\u00EDc\u00EDch projekt\u016F, ke studiu mechanick\u00FDch vlastnost\u00ED a uspo\u0159\u00E1d\u00E1n\u00ED interf\u00E1zn\u00EDho chromatinu v j\u00E1drech \u017Eiv\u00FDch i fixovan\u00FDch lidsk\u00FDch bun\u011Bk. Teoretick\u00E9 a experiment\u00E1ln\u00ED zku\u0161enosti interdisciplin\u00E1rn\u00EDho t\u00FDmu \u0159e\u0161itel\u016F budou vyu\u017Eity: - k fluorescen\u010Dn\u00EDmu barven\u00ED chromatinu \u017Eiv\u00FDch bun\u011Bk, - k laserem indukovan\u00E9 f\u00FAzi \u017Eiv\u00FDch fluorescen\u010Dn\u011B ozna\u010Den\u00FDch bun\u011Bk, - k topologick\u00E9 anal\u00FDze rozlo\u017Een\u00ED obarven\u00E9 oblasti chromatinu v j\u00E1drech \u017Eiv\u00FDch a fixovan\u00FDch bun\u011Bk, - ke vn\u00E1\u0161en\u00ED fluorescen\u010Dn\u011B obarven\u00FDch nanosond do j\u00E1dra \u017Eiv\u00E9 bu\u0148ky, optick\u00E9mu zachycen\u00ED sondy a vymezen\u00ED prostoru, kter\u00FD je pro sondu nedostupn\u00FD, s mikrometrov\u00FDm rozli\u0161en\u00EDm, - ke studiu mechanick\u00FDch vlastnost\u00ED chromatinu s vyu\u017Eit\u00EDm optick\u00FDch manipulac\u00ED s vhodn\u00FDmi sondami. Z\u00EDskan\u00E9 poznatky budou vyu\u017Eity pro v\u00FDvoj nov\u00FDch diagnostick\u00FDch metod."@cs . . . . . "4"^^ . . . "The use of combination of laser microbeam and cytometric techniques in the study of the structure and dynamics of human genome"@en . .