Nucleic Acid, Nucleoside, or NucleotideIndicator, Reagent, or Diagnostic Aid
nci:P108
DNA Probes
nci:P207
C0012893
nci:P325
<n0:ComplexDefinition xmlns:n0="http://ncicb.nci.nih.gov/xml/owl/EVS/ComplexProperties.xsd#"><n0:def-definition>Species- or subspecies-specific DNA (including COMPLEMENTARY DNA; conserved genes, whole chromosomes, or whole genomes) used in hybridization studies in order to identify microorganisms, to measure DNA-DNA homologies, to group subspecies, etc. The DNA probe hybridizes with a specific mRNA, if present. Conventional techniques used for testing for the hybridization product include dot blot assays, Southern blot assays, and DNA:RNA hybrid-specific antibody tests. Conventional labels for the DNA probe include the radioisotope labels 32P and 125I and the chemical label biotin. The use of DNA probes provides a specific, sensitive, rapid, and inexpensive replacement for cell culture techniques for diagnosing infections.</n0:def-definition><n0:def-source>MSH2002_06_01</n0:def-source></n0:ComplexDefinition>
<n0:ComplexDefinition xmlns:n0="http://ncicb.nci.nih.gov/xml/owl/EVS/ComplexProperties.xsd#"><n0:def-definition>A piece of DNA that has been labeled, usually radioactively or with a fluorescent dye, which is used in hybridization studies. Applications include Northern and Southern blots, in situ hybridization techniques, and diagnostic tests. DNA probes can be highly specific or degenerate.</n0:def-definition><n0:def-source>NCI</n0:def-source></n0:ComplexDefinition>