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Linkomycin je antibiotikum účinné proti grampozitivním aerobním a anaerobním bakteriím včetně Streptococcus pyogenes, S. pneumoniae, S. viridans , Staphylococcus aureus (včetně penicilin rezistentních kmenů), Corynebacterium diphtheriae, Bacillus anthracis , Nocardia spp., některým Clostridium species, Peptococcus, Peptostreptococcus, Eubacterium, Propiobacterium, Bacterioides fragilis a Actinomyces israelii.