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Statements

Subject Item
n2:RIV%2F86652036%3A_____%2F13%3A00394256%21RIV14-AV0-86652036
rdf:type
n17:Vysledek skos:Concept
dcterms:description
One of the most important requirements to get good results from qPCR experiments is to have a template of good quality. In most cases this means having an efficient sample preparation. This course module is focused on extraction of RNA and DNA to be used as template in qPCR and reverse transcription reactions. It also includes the important quality control steps needed to asure reliable results. The course avers : Overview of nucleic acid extraction methods. How to properly determine the concentration of purified nucleic acids. Storage of biological samples and purified nucleic acids. Quality control of purified material. Integrity of purified RNA. How to test for the presence of inhibitors. One of the most important requirements to get good results from qPCR experiments is to have a template of good quality. In most cases this means having an efficient sample preparation. This course module is focused on extraction of RNA and DNA to be used as template in qPCR and reverse transcription reactions. It also includes the important quality control steps needed to asure reliable results. The course avers : Overview of nucleic acid extraction methods. How to properly determine the concentration of purified nucleic acids. Storage of biological samples and purified nucleic acids. Quality control of purified material. Integrity of purified RNA. How to test for the presence of inhibitors.
dcterms:title
TATAA courses – 1 day Sample preparation and quality control of nucleic acids TATAA courses – 1 day Sample preparation and quality control of nucleic acids
skos:prefLabel
TATAA courses – 1 day Sample preparation and quality control of nucleic acids TATAA courses – 1 day Sample preparation and quality control of nucleic acids
skos:notation
RIV/86652036:_____/13:00394256!RIV14-AV0-86652036
n17:predkladatel
n19:ico%3A86652036
n3:aktivita
n9:Z
n3:aktivity
Z(AV0Z50520701)
n3:dodaniDat
n11:2014
n3:domaciTvurceVysledku
n12:2725258 n12:3757501 n12:6082920
n3:druhVysledku
n18:W
n3:duvernostUdaju
n4:S
n3:entitaPredkladatele
n14:predkladatel
n3:idSjednocenehoVysledku
109840
n3:idVysledku
RIV/86652036:_____/13:00394256
n3:jazykVysledku
n15:eng
n3:klicovaSlova
: qPCR; quality control; reverese transcription
n3:klicoveSlovo
n5:quality%20control n5:reverese%20transcription n5:%3A%20qPCR
n3:kontrolniKodProRIV
[63447CCFA03D]
n3:mistoKonaniAkce
Praha, BTÚ
n3:obor
n6:EB
n3:pocetDomacichTvurcuVysledku
3
n3:pocetTvurcuVysledku
3
n3:pocetUcastnikuAkce
5
n3:pocetZahranicnichUcastnikuAkce
3
n3:rokUplatneniVysledku
n11:2013
n3:statKonaniAkce
n16:CZ
n3:tvurceVysledku
Kubista, Mikael Flachsová, Monika Jindřichová, Marie
n3:typAkce
n7:WRD
n3:ukonceniAkce
2013-06-26+02:00
n3:zahajeniAkce
2013-06-26+02:00
n3:zamer
n8:AV0Z50520701