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Statements

Subject Item
n2:RIV%2F86652036%3A_____%2F13%3A00394088%21RIV14-AV0-86652036
rdf:type
n8:Vysledek skos:Concept
dcterms:description
Day 1 - This introductory course consists of a theoretical part and a practical part where participants get to do qPCR experiments by themselves under experienced supervision. The course contains: Basic PCR theory. The theory of real-time PCR. Applications and possibilities of qPCR. Comparison of qPCR with regular PCR. Review of currently available detection technologies (SYBR Green I, hydrolysis probes, Molecular Beacons...etc). Different instrument platforms and their typical uses. Primer design. The problem of primer-dimer formation and how to minimize them. Probe design. Experimental design and optimization. Basic data handling and analysis. Day 2 - Introduction to quantification principles. Quantification strategies, uses and limitations. Calculations using different relative quantification methods. Strategies for normalization of qPCR data. In situ calibration for compensation of inhibition in samples. Absolute quantification. Day 1 - This introductory course consists of a theoretical part and a practical part where participants get to do qPCR experiments by themselves under experienced supervision. The course contains: Basic PCR theory. The theory of real-time PCR. Applications and possibilities of qPCR. Comparison of qPCR with regular PCR. Review of currently available detection technologies (SYBR Green I, hydrolysis probes, Molecular Beacons...etc). Different instrument platforms and their typical uses. Primer design. The problem of primer-dimer formation and how to minimize them. Probe design. Experimental design and optimization. Basic data handling and analysis. Day 2 - Introduction to quantification principles. Quantification strategies, uses and limitations. Calculations using different relative quantification methods. Strategies for normalization of qPCR data. In situ calibration for compensation of inhibition in samples. Absolute quantification.
dcterms:title
TATAA courses – 2 days Hands-on qPCR TATAA courses – 2 days Hands-on qPCR
skos:prefLabel
TATAA courses – 2 days Hands-on qPCR TATAA courses – 2 days Hands-on qPCR
skos:notation
RIV/86652036:_____/13:00394088!RIV14-AV0-86652036
n8:predkladatel
n16:ico%3A86652036
n4:aktivita
n11:Z
n4:aktivity
Z(AV0Z50520701)
n4:dodaniDat
n19:2014
n4:domaciTvurceVysledku
n5:3757501 n5:3982815 n5:6082920 n5:1092030 n5:2725258
n4:druhVysledku
n14:W
n4:duvernostUdaju
n10:S
n4:entitaPredkladatele
n9:predkladatel
n4:idSjednocenehoVysledku
109846
n4:idVysledku
RIV/86652036:_____/13:00394088
n4:jazykVysledku
n18:eng
n4:klicovaSlova
qPCR; biostatistic; reverese transcription
n4:klicoveSlovo
n6:qPCR n6:reverese%20transcription n6:biostatistic
n4:kontrolniKodProRIV
[D6774B60AE7C]
n4:mistoKonaniAkce
Praha, BTÚ
n4:obor
n13:EB
n4:pocetDomacichTvurcuVysledku
5
n4:pocetTvurcuVysledku
5
n4:pocetUcastnikuAkce
10
n4:pocetZahranicnichUcastnikuAkce
5
n4:rokUplatneniVysledku
n19:2013
n4:statKonaniAkce
n17:CZ
n4:tvurceVysledku
Korenková, Vlasta Valihrach, Lukáš Jindřichová, Marie Kubista, Mikael Flachsová, Monika
n4:typAkce
n15:WRD
n4:ukonceniAkce
2013-03-05+01:00
n4:zahajeniAkce
2013-03-04+01:00
n4:zamer
n12:AV0Z50520701