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Statements

Subject Item
n2:RIV%2F86652036%3A_____%2F13%3A00394086%21RIV14-AV0-86652036
rdf:type
n5:Vysledek skos:Concept
dcterms:description
Day 1 - This introductory course consists of a theoretical part and a practical part where participants get to do qPCR experiments by themselves under experienced supervision. The course contains: Basic PCR theory. The theory of real-time PCR. Applications and possibilities of qPCR. Comparison of qPCR with regular PCR. Review of currently available detection technologies (SYBR Green I, hydrolysis probes, Molecular Beacons...etc). Different instrument platforms and their typical uses. Primer design. The problem of primer-dimer formation and how to minimize them. Probe design. Experimental design and optimization. Basic data handling and analysis. Day 2 - Introduction to quantification principles. Quantification strategies, uses and limitations. Calculations using different relative quantification methods. Strategies for normalization of qPCR data. In situ calibration for compensation of inhibition in samples. Absolute quantification. Day 3 - Sample Preparation (Extraction of RNA and DNA). Principles of RT. Priming methods for RT. RT optimization. The MIQE guidelines. Group discussion, bring your own questions. Day 1 - This introductory course consists of a theoretical part and a practical part where participants get to do qPCR experiments by themselves under experienced supervision. The course contains: Basic PCR theory. The theory of real-time PCR. Applications and possibilities of qPCR. Comparison of qPCR with regular PCR. Review of currently available detection technologies (SYBR Green I, hydrolysis probes, Molecular Beacons...etc). Different instrument platforms and their typical uses. Primer design. The problem of primer-dimer formation and how to minimize them. Probe design. Experimental design and optimization. Basic data handling and analysis. Day 2 - Introduction to quantification principles. Quantification strategies, uses and limitations. Calculations using different relative quantification methods. Strategies for normalization of qPCR data. In situ calibration for compensation of inhibition in samples. Absolute quantification. Day 3 - Sample Preparation (Extraction of RNA and DNA). Principles of RT. Priming methods for RT. RT optimization. The MIQE guidelines. Group discussion, bring your own questions.
dcterms:title
TATAA courses : 3 days Hands-on qPCR TATAA courses : 3 days Hands-on qPCR
skos:prefLabel
TATAA courses : 3 days Hands-on qPCR TATAA courses : 3 days Hands-on qPCR
skos:notation
RIV/86652036:_____/13:00394086!RIV14-AV0-86652036
n5:predkladatel
n16:ico%3A86652036
n3:aktivita
n9:Z
n3:aktivity
Z(AV0Z50520701)
n3:dodaniDat
n19:2014
n3:domaciTvurceVysledku
n4:3757501 n4:6082920 n4:8174326 n4:1092030
n3:druhVysledku
n12:W
n3:duvernostUdaju
n10:S
n3:entitaPredkladatele
n7:predkladatel
n3:idSjednocenehoVysledku
109848
n3:idVysledku
RIV/86652036:_____/13:00394086
n3:jazykVysledku
n18:eng
n3:klicovaSlova
qPCR; biostatistic; reverese transcription
n3:klicoveSlovo
n6:qPCR n6:reverese%20transcription n6:biostatistic
n3:kontrolniKodProRIV
[C5C2C5F24E6E]
n3:mistoKonaniAkce
Praha, BTÚ
n3:obor
n11:EB
n3:pocetDomacichTvurcuVysledku
4
n3:pocetTvurcuVysledku
4
n3:pocetUcastnikuAkce
11
n3:pocetZahranicnichUcastnikuAkce
1
n3:rokUplatneniVysledku
n19:2013
n3:statKonaniAkce
n17:CZ
n3:tvurceVysledku
Kubista, Mikael Flachsová, Monika Mikulová, Veronika Korenková, Vlasta
n3:typAkce
n13:WRD
n3:ukonceniAkce
2013-02-06+01:00
n3:zahajeniAkce
2013-02-04+01:00
n3:zamer
n15:AV0Z50520701