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Statements

Subject Item
n2:RIV%2F86652036%3A_____%2F13%3A00392024%21RIV14-AV0-86652036
rdf:type
skos:Concept n18:Vysledek
dcterms:description
The aim of this study was to trigger the expression of genes related to oocytes in putative ovarian stem cells scraped from the ovarian surface epithelium of women with premature ovarian failure and cultured in vitro in the presence of follicular fluid, rich in substances for oocyte growth and maturation. Ovarian surface epithelium was scraped and cell cultures were set up by scrapings in five women with nonfunctional ovaries and with no naturally present mature follicles or oocytes. In the presence of donated follicular fluid putative stem cells grew and developed into primitive oocyte-like cells. A detailed single-cell gene expression profiling was performed to elucidate their genetic status in comparison to human embryonic stem cells, oocytes, and somatic fibroblasts. The ovarian cell cultures depleted/converted reproductive hormones from the culture medium. Estradiol alone or together with other substances may be involved in development of these primitive oocyte-like cells. The majority of primitive oocyte-like cells was mononuclear and expressed several genes related to pluripotency and oocytes, including genes related to meiosis, although they did not express some important oocyte-specific genes. Our work reveals the presence of putative stem cells in the ovarian surface epithelium of women with premature ovarian failure. The aim of this study was to trigger the expression of genes related to oocytes in putative ovarian stem cells scraped from the ovarian surface epithelium of women with premature ovarian failure and cultured in vitro in the presence of follicular fluid, rich in substances for oocyte growth and maturation. Ovarian surface epithelium was scraped and cell cultures were set up by scrapings in five women with nonfunctional ovaries and with no naturally present mature follicles or oocytes. In the presence of donated follicular fluid putative stem cells grew and developed into primitive oocyte-like cells. A detailed single-cell gene expression profiling was performed to elucidate their genetic status in comparison to human embryonic stem cells, oocytes, and somatic fibroblasts. The ovarian cell cultures depleted/converted reproductive hormones from the culture medium. Estradiol alone or together with other substances may be involved in development of these primitive oocyte-like cells. The majority of primitive oocyte-like cells was mononuclear and expressed several genes related to pluripotency and oocytes, including genes related to meiosis, although they did not express some important oocyte-specific genes. Our work reveals the presence of putative stem cells in the ovarian surface epithelium of women with premature ovarian failure.
dcterms:title
Expression of Pluripotency and Oocyte-Related Genes in Single Putative Stem Cells from Human Adult Ovarian Surface Epithelium Cultured In Vitro in the Presence of Follicular Fluid Expression of Pluripotency and Oocyte-Related Genes in Single Putative Stem Cells from Human Adult Ovarian Surface Epithelium Cultured In Vitro in the Presence of Follicular Fluid
skos:prefLabel
Expression of Pluripotency and Oocyte-Related Genes in Single Putative Stem Cells from Human Adult Ovarian Surface Epithelium Cultured In Vitro in the Presence of Follicular Fluid Expression of Pluripotency and Oocyte-Related Genes in Single Putative Stem Cells from Human Adult Ovarian Surface Epithelium Cultured In Vitro in the Presence of Follicular Fluid
skos:notation
RIV/86652036:_____/13:00392024!RIV14-AV0-86652036
n18:predkladatel
n19:ico%3A86652036
n3:aktivita
n4:Z
n3:aktivity
Z(AV0Z50520701)
n3:cisloPeriodika
861460
n3:dodaniDat
n11:2014
n3:domaciTvurceVysledku
n12:3757501
n3:druhVysledku
n15:J
n3:duvernostUdaju
n16:S
n3:entitaPredkladatele
n17:predkladatel
n3:idSjednocenehoVysledku
74354
n3:idVysledku
RIV/86652036:_____/13:00392024
n3:jazykVysledku
n10:eng
n3:klicovaSlova
single cell; gene expression profiling; oocytes
n3:klicoveSlovo
n5:gene%20expression%20profiling n5:oocytes n5:single%20cell
n3:kodStatuVydavatele
US - Spojené státy americké
n3:kontrolniKodProRIV
[2200F33385F3]
n3:nazevZdroje
BIOMED RESEARCH INTERNATIONAL
n3:obor
n13:EB
n3:pocetDomacichTvurcuVysledku
1
n3:pocetTvurcuVysledku
6
n3:rokUplatneniVysledku
n11:2013
n3:tvurceVysledku
Skutella, T. Vogler, A. Virant-Klun, I. Sinkovec, J. Kubista, Mikael Meden-Vrtovec, H.
n3:wos
000315880200001
n3:zamer
n8:AV0Z50520701
s:issn
2314-6133
s:numberOfPages
18
n14:doi
10.1155/2013/861460