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Statements

Subject Item
n2:RIV%2F86652036%3A_____%2F12%3A00389225%21RIV13-AV0-86652036
rdf:type
n5:Vysledek skos:Concept
dcterms:description
One of the most important requirements to get good results from qPCR experiments is to have a template of good quality. In most cases this means having an efficient sample preparation. This course module is focused on extraction of RNA and DNA to be used as template in qPCR and reverse transcription reactions. It also includes the important quality control steps needed to asure reliable results. The course avers : Overview of nucleic acid extraction methods. How to properly determine the concentration of purified nucleic acids. Storage of biological samples and purified nucleic acids. Quality control of purified material. Integrity of purified RNA. How to test for the presence of inhibitors. One of the most important requirements to get good results from qPCR experiments is to have a template of good quality. In most cases this means having an efficient sample preparation. This course module is focused on extraction of RNA and DNA to be used as template in qPCR and reverse transcription reactions. It also includes the important quality control steps needed to asure reliable results. The course avers : Overview of nucleic acid extraction methods. How to properly determine the concentration of purified nucleic acids. Storage of biological samples and purified nucleic acids. Quality control of purified material. Integrity of purified RNA. How to test for the presence of inhibitors.
dcterms:title
1 day Sample preparation and quality control of nucleic acids 1 day Sample preparation and quality control of nucleic acids
skos:prefLabel
1 day Sample preparation and quality control of nucleic acids 1 day Sample preparation and quality control of nucleic acids
skos:notation
RIV/86652036:_____/12:00389225!RIV13-AV0-86652036
n5:predkladatel
n9:ico%3A86652036
n3:aktivita
n11:Z
n3:aktivity
Z(AV0Z50520701)
n3:dodaniDat
n4:2013
n3:domaciTvurceVysledku
n15:9313605 n15:3757501
n3:druhVysledku
n6:W
n3:duvernostUdaju
n7:S
n3:entitaPredkladatele
n17:predkladatel
n3:idSjednocenehoVysledku
183209
n3:idVysledku
RIV/86652036:_____/12:00389225
n3:jazykVysledku
n16:eng
n3:klicovaSlova
qPCR; quality control; reverese transcription
n3:klicoveSlovo
n8:reverese%20transcription n8:quality%20control n8:qPCR
n3:kontrolniKodProRIV
[864327670A6F]
n3:mistoKonaniAkce
Praha, BTÚ
n3:obor
n12:EB
n3:pocetDomacichTvurcuVysledku
2
n3:pocetTvurcuVysledku
2
n3:pocetUcastnikuAkce
9
n3:pocetZahranicnichUcastnikuAkce
3
n3:rokUplatneniVysledku
n4:2012
n3:statKonaniAkce
n18:CZ
n3:tvurceVysledku
Kubista, Mikael Kološtová, Katarina
n3:typAkce
n14:WRD
n3:ukonceniAkce
2012-10-24+02:00
n3:zahajeniAkce
2012-10-24+02:00
n3:zamer
n19:AV0Z50520701