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Statements

Subject Item
n2:RIV%2F75010330%3A_____%2F12%3A00009802%21RIV13-GA0-75010330
rdf:type
n4:Vysledek skos:Concept
dcterms:description
Aims: To charactinosa in order to evaluate their potential for use as biomarkers of lung infection in noninvasive breath analysis. Methods and Results: Volatile organic compounds (VOCs) emitted from 36 clinical strains of Ps. aeruginosa (belonging to different multilocus sequence types) cultured in liquid and on solid media were analysed by gas chromatography mass spectrometry (GC-MS) and selected ion flow tube mass spectrometry (SIFT-MS). Several previously identified VOCs were detected, including ethanol, acetone, 2-butanone, 2-pentanone, isoprene, aminoacetophenone, dimethyl sulphide, dimethyl disulphide, dimethyl trisulphide and methyl thiocyanate. Additionally, significant production of 3-methyl-butanone, acetophenone, methylthioacetate and methyl thiobutanoate was observed for the first time in this study. SIFT-MS quantifications of VOCs showed high variability between genotypically distinct strains. Conclusions: The data obtained indicate that the production rates of the volatile biomarkers of. Aims: To charactinosa in order to evaluate their potential for use as biomarkers of lung infection in noninvasive breath analysis. Methods and Results: Volatile organic compounds (VOCs) emitted from 36 clinical strains of Ps. aeruginosa (belonging to different multilocus sequence types) cultured in liquid and on solid media were analysed by gas chromatography mass spectrometry (GC-MS) and selected ion flow tube mass spectrometry (SIFT-MS). Several previously identified VOCs were detected, including ethanol, acetone, 2-butanone, 2-pentanone, isoprene, aminoacetophenone, dimethyl sulphide, dimethyl disulphide, dimethyl trisulphide and methyl thiocyanate. Additionally, significant production of 3-methyl-butanone, acetophenone, methylthioacetate and methyl thiobutanoate was observed for the first time in this study. SIFT-MS quantifications of VOCs showed high variability between genotypically distinct strains. Conclusions: The data obtained indicate that the production rates of the volatile biomarkers of.
dcterms:title
Variability in concentrations of volatile metabolites emitted by genotypically different strains of Pseudomonas aeruginosa Variability in concentrations of volatile metabolites emitted by genotypically different strains of Pseudomonas aeruginosa
skos:prefLabel
Variability in concentrations of volatile metabolites emitted by genotypically different strains of Pseudomonas aeruginosa Variability in concentrations of volatile metabolites emitted by genotypically different strains of Pseudomonas aeruginosa
skos:notation
RIV/75010330:_____/12:00009802!RIV13-GA0-75010330
n4:predkladatel
n8:ico%3A75010330
n5:aktivita
n14:I n14:P
n5:aktivity
I, P(GA203/09/0256)
n5:cisloPeriodika
3
n5:dodaniDat
n10:2013
n5:domaciTvurceVysledku
n15:1892436 n15:8880174
n5:druhVysledku
n12:J
n5:duvernostUdaju
n7:S
n5:entitaPredkladatele
n17:predkladatel
n5:idSjednocenehoVysledku
176991
n5:idVysledku
RIV/75010330:_____/12:00009802
n5:jazykVysledku
n19:eng
n5:klicovaSlova
Pseudomonas aeruginosa; in vitro; genotyping; MLST; GC/MS; SIFT-MS
n5:klicoveSlovo
n6:Pseudomonas%20aeruginosa n6:genotyping n6:MLST n6:in%20vitro n6:SIFT-MS n6:GC%2FMS
n5:kodStatuVydavatele
GB - Spojené království Velké Británie a Severního Irska
n5:kontrolniKodProRIV
[93699150D9B5]
n5:nazevZdroje
Journal of applied microbiology
n5:obor
n18:EE
n5:pocetDomacichTvurcuVysledku
2
n5:pocetTvurcuVysledku
7
n5:projekt
n16:GA203%2F09%2F0256
n5:rokUplatneniVysledku
n10:2012
n5:svazekPeriodika
113
n5:tvurceVysledku
Shestivska, V. Španěl, P. Dryahina, K. Nemec, Alexandr Musílek, Martin Smith, D. Sovová, K.
n5:wos
000307558800025
s:issn
1364-5072
s:numberOfPages
13
n9:doi
10.1111/j.1365-2672.2012.05370.x