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Statements

Subject Item
n2:RIV%2F75010330%3A_____%2F12%3A00009617%21RIV13-MZ0-75010330
rdf:type
n6:Vysledek skos:Concept
dcterms:description
Histologically verified pairs (n=10) of pancreatic tumors and non-neoplastic tissues were used for quantitative real-time PCR and the stability of 24 reference genes was analyzed with geNorm and NormFinder software. Raw C{q} values correlated with the degree of RNA degradation. This correlation was abolished by normalization to C{q} of 18S endogenous control gene. Both geNorm and NormFinder programs suggested EIF2B1, ELF1, MRPL19, and POP4 as the same most stable genes. We have thus identified suitable reference genes for future expression studies in pancreatic carcinoma. Normalization method reducing the effects of RNA degradation on the quality of results was also developed. Histologically verified pairs (n=10) of pancreatic tumors and non-neoplastic tissues were used for quantitative real-time PCR and the stability of 24 reference genes was analyzed with geNorm and NormFinder software. Raw C{q} values correlated with the degree of RNA degradation. This correlation was abolished by normalization to C{q} of 18S endogenous control gene. Both geNorm and NormFinder programs suggested EIF2B1, ELF1, MRPL19, and POP4 as the same most stable genes. We have thus identified suitable reference genes for future expression studies in pancreatic carcinoma. Normalization method reducing the effects of RNA degradation on the quality of results was also developed.
dcterms:title
Evaluation of reference genes and normalization strategy for quantitative real-time PCR in human pancreatic carcinoma Evaluation of reference genes and normalization strategy for quantitative real-time PCR in human pancreatic carcinoma
skos:prefLabel
Evaluation of reference genes and normalization strategy for quantitative real-time PCR in human pancreatic carcinoma Evaluation of reference genes and normalization strategy for quantitative real-time PCR in human pancreatic carcinoma
skos:notation
RIV/75010330:_____/12:00009617!RIV13-MZ0-75010330
n6:predkladatel
n14:ico%3A75010330
n3:aktivita
n16:P n16:V
n3:aktivity
P(NS10230), P(NS9799), V
n3:cisloPeriodika
3
n3:dodaniDat
n15:2013
n3:domaciTvurceVysledku
n11:4078829 n11:9595007
n3:druhVysledku
n10:J
n3:duvernostUdaju
n18:S
n3:entitaPredkladatele
n13:predkladatel
n3:idSjednocenehoVysledku
135161
n3:idVysledku
RIV/75010330:_____/12:00009617
n3:jazykVysledku
n7:eng
n3:klicovaSlova
pancreas carcinoma; pancreas; carcinoma; PCR; RNA
n3:klicoveSlovo
n8:RNA n8:pancreas%20carcinoma n8:PCR n8:carcinoma n8:pancreas
n3:kodStatuVydavatele
NL - Nizozemsko
n3:kontrolniKodProRIV
[484F2B721EC6]
n3:nazevZdroje
Disease markers
n3:obor
n4:EB
n3:pocetDomacichTvurcuVysledku
2
n3:pocetTvurcuVysledku
4
n3:projekt
n17:NS10230 n17:NS9799
n3:rokUplatneniVysledku
n15:2012
n3:svazekPeriodika
32
n3:tvurceVysledku
Mohelníková-Duchoňová, Beatrice Honsová, E. Souček, Pavel Oliverius, M.
n3:wos
000301045600008
s:issn
0278-0240
s:numberOfPages
8