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Statements

Subject Item
n2:RIV%2F75010330%3A_____%2F10%3A00008185%21RIV11-MZ0-75010330
rdf:type
n6:Vysledek skos:Concept
dcterms:description
Washed red blood cells (RBCs), supplemented or non-supplemented with sodium azide (to inhibit catalase activity), were exposed to different concentrations of hydrogen peroxide as well as ascorbic acid. Strikingly, catalase within RBCs protected the cells against exogenic hydrogen peroxide even at millimolar concentrations. However, the activity of the erythrocytic catalase failed to protect the RBCs when they were exposed to an oxidative burst of stimulated polymorphonuclear cells (PMNCs) in the presence of several reactive species in addition to peroxide. Oxyhemoglobin, with an excess of hydrogen peroxide, formed oxidized hemoglobin species and caused protein denaturation as well as the rise of heme degradation products which was suspected to falsify zinc protoporphyrin/heme (ZPP/heme) ratio as assessed by hematofluorometry. Our experiments may thus imply that the non-fluorescent hemoglobin background can be modified by reactive oxygen species (ROS) and this can lead to a spurious ZPP/heme ratio. Washed red blood cells (RBCs), supplemented or non-supplemented with sodium azide (to inhibit catalase activity), were exposed to different concentrations of hydrogen peroxide as well as ascorbic acid. Strikingly, catalase within RBCs protected the cells against exogenic hydrogen peroxide even at millimolar concentrations. However, the activity of the erythrocytic catalase failed to protect the RBCs when they were exposed to an oxidative burst of stimulated polymorphonuclear cells (PMNCs) in the presence of several reactive species in addition to peroxide. Oxyhemoglobin, with an excess of hydrogen peroxide, formed oxidized hemoglobin species and caused protein denaturation as well as the rise of heme degradation products which was suspected to falsify zinc protoporphyrin/heme (ZPP/heme) ratio as assessed by hematofluorometry. Our experiments may thus imply that the non-fluorescent hemoglobin background can be modified by reactive oxygen species (ROS) and this can lead to a spurious ZPP/heme ratio.
dcterms:title
Oxidative stress may modify zinc protoporphyrin/heme ratio in hematofluorometry Oxidative stress may modify zinc protoporphyrin/heme ratio in hematofluorometry
skos:prefLabel
Oxidative stress may modify zinc protoporphyrin/heme ratio in hematofluorometry Oxidative stress may modify zinc protoporphyrin/heme ratio in hematofluorometry
skos:notation
RIV/75010330:_____/10:00008185!RIV11-MZ0-75010330
n3:aktivita
n14:P
n3:aktivity
P(NS9802)
n3:cisloPeriodika
1p2
n3:dodaniDat
n17:2011
n3:domaciTvurceVysledku
n13:8964297 n13:5423953 n13:9962999
n3:druhVysledku
n11:J
n3:duvernostUdaju
n9:S
n3:entitaPredkladatele
n4:predkladatel
n3:idSjednocenehoVysledku
277879
n3:idVysledku
RIV/75010330:_____/10:00008185
n3:jazykVysledku
n16:eng
n3:klicovaSlova
oxidative stress; zinc-protoporphyrin
n3:klicoveSlovo
n5:zinc-protoporphyrin n5:oxidative%20stress
n3:kodStatuVydavatele
GB - Spojené království Velké Británie a Severního Irska
n3:kontrolniKodProRIV
[DC904213FD73]
n3:nazevZdroje
International journal of laboratory hematology
n3:obor
n15:FN
n3:pocetDomacichTvurcuVysledku
3
n3:pocetTvurcuVysledku
5
n3:projekt
n12:NS9802
n3:rokUplatneniVysledku
n17:2010
n3:svazekPeriodika
32
n3:tvurceVysledku
Jírová, Dagmar Janoušek, Stanislav Rosa, L. Kejlová, Kristina Janoušek, S. J.
n3:wos
000275511300006
s:issn
1751-5521
s:numberOfPages
10