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Statements

Subject Item
n2:RIV%2F70883521%3A28610%2F14%3A43871817%21RIV15-MSM-28610___
rdf:type
n17:Vysledek skos:Concept
rdfs:seeAlso
http://www.sciencedirect.com/science/article/pii/S0927775713009394
dcterms:description
In the last few decades, there has been a growing interest towards the use of delivery systems for a more effective treatment of various diseases and the research increasingly focused on designing innovative solutions based on intra-cellular vectors. Among them, biocompatible DNA-polyelectrolyte complexes appear as a promising strategy for in vivo delivery of biologically active macromolecules. One of the most largely employed cationic polymer is Chitosan, which is special for its biological properties such as biodegradability, biocompatibility, mucoadhesivity, and permeability enhancer capacity. Due to this, complexes formed by condensation of DNA by Chitosan have been largely investigated for their potential use in gene therapy. Nevertheless the extensive efforts, the correlation between the physicochemical properties of the Chitosan-DNA polyplexes with their transfection efficiency still remains a central challenge. Moreover, the criteria and strategies for the design of efficient Chitosan-based gene delivery systems remain inconclusive. In a recent paper, we studied the aggregation behavior of Chitosan-DNA complexes and compared it with the predictions of existing models for the complexation of oppositely charged polyelectrolytes, showing that these models can serve as useful guide for the optimization of the complexes. Here, in order to understand the relation between physicochemical and transfection properties of Chitosan-DNA complexes, we study the efficiency of Chitosan-pDNA aggregates obtained indifferent conditions as vectors for DNA transfection. Small, globular and positively charged aggregates formed at large Chitosan excess, which can be obtained independently of the length of the Chitosan employed, appear to be the more effective for transfection, the more stable aggregates resulting the ones formed with longer chains. In the last few decades, there has been a growing interest towards the use of delivery systems for a more effective treatment of various diseases and the research increasingly focused on designing innovative solutions based on intra-cellular vectors. Among them, biocompatible DNA-polyelectrolyte complexes appear as a promising strategy for in vivo delivery of biologically active macromolecules. One of the most largely employed cationic polymer is Chitosan, which is special for its biological properties such as biodegradability, biocompatibility, mucoadhesivity, and permeability enhancer capacity. Due to this, complexes formed by condensation of DNA by Chitosan have been largely investigated for their potential use in gene therapy. Nevertheless the extensive efforts, the correlation between the physicochemical properties of the Chitosan-DNA polyplexes with their transfection efficiency still remains a central challenge. Moreover, the criteria and strategies for the design of efficient Chitosan-based gene delivery systems remain inconclusive. In a recent paper, we studied the aggregation behavior of Chitosan-DNA complexes and compared it with the predictions of existing models for the complexation of oppositely charged polyelectrolytes, showing that these models can serve as useful guide for the optimization of the complexes. Here, in order to understand the relation between physicochemical and transfection properties of Chitosan-DNA complexes, we study the efficiency of Chitosan-pDNA aggregates obtained indifferent conditions as vectors for DNA transfection. Small, globular and positively charged aggregates formed at large Chitosan excess, which can be obtained independently of the length of the Chitosan employed, appear to be the more effective for transfection, the more stable aggregates resulting the ones formed with longer chains.
dcterms:title
Chitosan-DNA complexes: Effect of molecular parameters on the efficiency of delivery Chitosan-DNA complexes: Effect of molecular parameters on the efficiency of delivery
skos:prefLabel
Chitosan-DNA complexes: Effect of molecular parameters on the efficiency of delivery Chitosan-DNA complexes: Effect of molecular parameters on the efficiency of delivery
skos:notation
RIV/70883521:28610/14:43871817!RIV15-MSM-28610___
n4:aktivita
n13:V
n4:aktivity
V
n4:cisloPeriodika
Neuveden
n4:dodaniDat
n10:2015
n4:domaciTvurceVysledku
Di Martino, Antonio
n4:druhVysledku
n18:J
n4:duvernostUdaju
n15:S
n4:entitaPredkladatele
n6:predkladatel
n4:idSjednocenehoVysledku
7169
n4:idVysledku
RIV/70883521:28610/14:43871817
n4:jazykVysledku
n9:eng
n4:klicovaSlova
Transfection; Gene-therapy; Polyplexes; Polyelectrolytes; Chitosan; DNA
n4:klicoveSlovo
n12:Chitosan n12:Polyplexes n12:Gene-therapy n12:DNA n12:Transfection n12:Polyelectrolytes
n4:kodStatuVydavatele
NL - Nizozemsko
n4:kontrolniKodProRIV
[27B62AF0870F]
n4:nazevZdroje
Colloids and Surfaces A: Physicochemical and Engineering Aspects
n4:obor
n14:CD
n4:pocetDomacichTvurcuVysledku
1
n4:pocetTvurcuVysledku
9
n4:rokUplatneniVysledku
n10:2014
n4:svazekPeriodika
460
n4:tvurceVysledku
Palocci, Cleofe Cifani, Noemi Bordi, Federico Ascenzioni, Fiorentina Pompili, Barbara Sennato, Simona Chronopoulou, Laura Bomboi, Francesca Di Martino, Antonio
n4:wos
000341880200025
s:issn
0927-7757
s:numberOfPages
7
n16:doi
10.1016/j.colsurfa.2013.12.022
n11:organizacniJednotka
28610