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Statements

Subject Item
n2:RIV%2F70883521%3A28610%2F14%3A43871809%21RIV15-MSM-28610___
rdf:type
skos:Concept n19:Vysledek
dcterms:description
Preparation of keratin hydrolyzates through alkaline-enzymatic hydrolysis was already described earlier. Employment of hydrolyzates in practice requires knowledge of their composition, thermal stability and molecular weight. The aim of this contribution is to determine molecular weight of keratin hydrolyzates (prepared from sheep wool). Molecular weight (Mw) of keratin hydrolyzates was determined by SDS-PAGE (sodium dodecyl sulphate polyacrylamide gel electrophoresis). Selection of hydrolysis conditions allows to prepare hydrolyzates with predominant share of low-molecular fractions, intermediate-molecular fractions or high-molecular fractions. Optimal conditions for preparing keratin hydrolyzates are as follows: in 1ststage of hydrolysis, acting on wool with 0.6% KOH at 90 °C for 48 h; in 2ndstage of hydrolysis, adding 1% (w/w) proteolytic enzyme Savinase 6 T and stirring at 40°C for 24 h. Under these conditions, breakdown efficiency is 63% and a hydrolyzate is prepared containing 11.5% N, 14.9% ash, 2.58% sulphur and a high portion of intermediate- and high-molecular fractions which is comparable in quality to technical gelatine. Keratin hydrolyzate can be employed, for example, to produce wrapping materials (films, coatings, encapsulates). Preparation of keratin hydrolyzates through alkaline-enzymatic hydrolysis was already described earlier. Employment of hydrolyzates in practice requires knowledge of their composition, thermal stability and molecular weight. The aim of this contribution is to determine molecular weight of keratin hydrolyzates (prepared from sheep wool). Molecular weight (Mw) of keratin hydrolyzates was determined by SDS-PAGE (sodium dodecyl sulphate polyacrylamide gel electrophoresis). Selection of hydrolysis conditions allows to prepare hydrolyzates with predominant share of low-molecular fractions, intermediate-molecular fractions or high-molecular fractions. Optimal conditions for preparing keratin hydrolyzates are as follows: in 1ststage of hydrolysis, acting on wool with 0.6% KOH at 90 °C for 48 h; in 2ndstage of hydrolysis, adding 1% (w/w) proteolytic enzyme Savinase 6 T and stirring at 40°C for 24 h. Under these conditions, breakdown efficiency is 63% and a hydrolyzate is prepared containing 11.5% N, 14.9% ash, 2.58% sulphur and a high portion of intermediate- and high-molecular fractions which is comparable in quality to technical gelatine. Keratin hydrolyzate can be employed, for example, to produce wrapping materials (films, coatings, encapsulates).
dcterms:title
Characterization of keratin hydrolyzates prepared from sheep wool Characterization of keratin hydrolyzates prepared from sheep wool
skos:prefLabel
Characterization of keratin hydrolyzates prepared from sheep wool Characterization of keratin hydrolyzates prepared from sheep wool
skos:notation
RIV/70883521:28610/14:43871809!RIV15-MSM-28610___
n4:aktivita
n13:P
n4:aktivity
P(ED2.1.00/03.0111)
n4:cisloPeriodika
19
n4:dodaniDat
n9:2015
n4:domaciTvurceVysledku
n16:9978429 n16:6067808
n4:druhVysledku
n14:J
n4:duvernostUdaju
n17:S
n4:entitaPredkladatele
n11:predkladatel
n4:idSjednocenehoVysledku
6949
n4:idVysledku
RIV/70883521:28610/14:43871809
n4:jazykVysledku
n8:eng
n4:klicovaSlova
Sheep wool; Molecular weight; Keratin; Hydrolyzate; Hydrolysis
n4:klicoveSlovo
n5:Keratin n5:Hydrolyzate n5:Molecular%20weight n5:Sheep%20wool n5:Hydrolysis
n4:kodStatuVydavatele
IN - Indická republika
n4:kontrolniKodProRIV
[6932929E0E9B]
n4:nazevZdroje
Asian Journal of Chemistry
n4:obor
n6:CD
n4:pocetDomacichTvurcuVysledku
2
n4:pocetTvurcuVysledku
4
n4:projekt
n15:ED2.1.00%2F03.0111
n4:rokUplatneniVysledku
n9:2014
n4:svazekPeriodika
26
n4:tvurceVysledku
Mokrejš, Pavel Sukop, Svatopluk Krejčí, Ondřej Svoboda, Petr
s:issn
0970-7077
s:numberOfPages
5
n12:doi
10.14233/ajchem.2014.16480
n10:organizacniJednotka
28610