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Statements

Subject Item
n2:RIV%2F70883521%3A28110%2F10%3A63510070%21RIV11-GA0-28110___
rdf:type
skos:Concept n16:Vysledek
dcterms:description
TGGE is an efficient method which allows us to study both composition and dynamics of complex microbial communities without the necessity of cultivation. Sample´s total DNA isolation and consequent PCR amplification of desired gene´s fragment need to be carried out before the TGGE experiment. Such pretreatment provides us with the population of gene fragments of the identical length from virtually all the strains present in the sample, and differing in their exact sequences. The DNA sequence variation in obtained gene fragments is a base for subsequent TGGE separation and identification of particular bacterial strains. Currently an equivalent method called denaturing gradient gel electrophoresis (DGGE) is used more often usually performed on D-code instrument (Bio-rad). In our laboratory we try to adopt TGGE instrumented on TGGE-Maxi system (Biometra) where the procedure is different particularly in the gel preparation and some other parameters. The instrument nowadays is relatively rare in the centra TGGE is an efficient method which allows us to study both composition and dynamics of complex microbial communities without the necessity of cultivation. Sample´s total DNA isolation and consequent PCR amplification of desired gene´s fragment need to be carried out before the TGGE experiment. Such pretreatment provides us with the population of gene fragments of the identical length from virtually all the strains present in the sample, and differing in their exact sequences. The DNA sequence variation in obtained gene fragments is a base for subsequent TGGE separation and identification of particular bacterial strains. Currently an equivalent method called denaturing gradient gel electrophoresis (DGGE) is used more often usually performed on D-code instrument (Bio-rad). In our laboratory we try to adopt TGGE instrumented on TGGE-Maxi system (Biometra) where the procedure is different particularly in the gel preparation and some other parameters. The instrument nowadays is relatively rare in the centra
dcterms:title
Temperature gradient gel electrophoresis (TGGE) in study of environmental samples: first experiences Temperature gradient gel electrophoresis (TGGE) in study of environmental samples: first experiences
skos:prefLabel
Temperature gradient gel electrophoresis (TGGE) in study of environmental samples: first experiences Temperature gradient gel electrophoresis (TGGE) in study of environmental samples: first experiences
skos:notation
RIV/70883521:28110/10:63510070!RIV11-GA0-28110___
n4:aktivita
n7:P
n4:aktivity
P(GAP108/10/0200)
n4:dodaniDat
n5:2011
n4:domaciTvurceVysledku
n8:4469143 n8:8588716 n8:8264945
n4:druhVysledku
n10:D
n4:duvernostUdaju
n19:S
n4:entitaPredkladatele
n14:predkladatel
n4:idSjednocenehoVysledku
292200
n4:idVysledku
RIV/70883521:28110/10:63510070
n4:jazykVysledku
n13:eng
n4:klicovaSlova
isolation of DNA; PCR; TGGE; reamplification of DNA
n4:klicoveSlovo
n9:reamplification%20of%20DNA n9:TGGE n9:PCR n9:isolation%20of%20DNA
n4:kontrolniKodProRIV
[704E97C64196]
n4:mistoKonaniAkce
Stará Lesná, Vysoké Tatry
n4:mistoVydani
Bratislava
n4:nazevZdroje
25. výročný kongres československej spoločnosti mikrobiologickej s medzinárodnou účasťou
n4:obor
n21:EE
n4:pocetDomacichTvurcuVysledku
3
n4:pocetTvurcuVysledku
3
n4:projekt
n11:GAP108%2F10%2F0200
n4:rokUplatneniVysledku
n5:2010
n4:tvurceVysledku
Buňková, Leona Koutný, Marek Husárová, Lucie
n4:typAkce
n20:EUR
n4:zahajeniAkce
2010-01-01+01:00
s:numberOfPages
1
n12:hasPublisher
Československá spoločnosť mikrobiologická
n18:isbn
970-80-970477-8-8
n17:organizacniJednotka
28110