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Statements

Subject Item
n2:RIV%2F70883521%3A28110%2F04%3A63502562%21RIV%2F2005%2FMSM%2F281105%2FN
rdf:type
n9:Vysledek skos:Concept
dcterms:description
In our study we aimed to find the universal procedure which would enable us to get DGGE profiles from larger set of samples where the soils differed substantially in contents of organic matter. Fraction of humic substances displays similar properties to DNA hence it can be uneasy to remove them during DNA isolation process and the substances consequently inhibit PCR. Additional cleaning of isolated DNA appears to be necessary. for most of our samples, two cleanings of isolated DNA (Wizard DNA clean-up systém, Promega) and subsequent tenfold dilution were sufficient. The most problematic sample needed three cleaning steps and tenfold dilution. Our experiences imply that dilution is more useful than additional cleaning for some samples. Byl ověřován postup isolace bakteriální DNA z velmi humosních stepních půd. Huminové látky ztěžovaly isolaci DNA z půd i inhibovaly následnou proceduru PCR. Práce prokázala, že dodatečné přečištění získané DNA je nezbytné. Z některých typů vzorků je však nemožné získat požadovanou DNA universálním postupem. In our study we aimed to find the universal procedure which would enable us to get DGGE profiles from larger set of samples where the soils differed substantially in contents of organic matter. Fraction of humic substances displays similar properties to DNA hence it can be uneasy to remove them during DNA isolation process and the substances consequently inhibit PCR. Additional cleaning of isolated DNA appears to be necessary. for most of our samples, two cleanings of isolated DNA (Wizard DNA clean-up systém, Promega) and subsequent tenfold dilution were sufficient. The most problematic sample needed three cleaning steps and tenfold dilution. Our experiences imply that dilution is more useful than additional cleaning for some samples.
dcterms:title
Isolation of Bacterial DNA from very Humic Steppe Soils and Subsequent DGGE Fingerprinting Isolace bakteriální DNA z velmi humosních stepních půd a následná DGGE Fingerprinting Isolation of Bacterial DNA from very Humic Steppe Soils and Subsequent DGGE Fingerprinting
skos:prefLabel
Isolation of Bacterial DNA from very Humic Steppe Soils and Subsequent DGGE Fingerprinting Isolace bakteriální DNA z velmi humosních stepních půd a následná DGGE Fingerprinting Isolation of Bacterial DNA from very Humic Steppe Soils and Subsequent DGGE Fingerprinting
skos:notation
RIV/70883521:28110/04:63502562!RIV/2005/MSM/281105/N
n3:strany
120-123
n3:aktivita
n10:Z
n3:aktivity
Z(MSM 281100002)
n3:dodaniDat
n18:2005
n3:domaciTvurceVysledku
n11:8588716
n3:druhVysledku
n15:D
n3:duvernostUdaju
n8:S
n3:entitaPredkladatele
n4:predkladatel
n3:idSjednocenehoVysledku
569074
n3:idVysledku
RIV/70883521:28110/04:63502562
n3:jazykVysledku
n19:eng
n3:klicovaSlova
bacterial DNA; isolation; soil; humic
n3:klicoveSlovo
n7:soil n7:humic n7:isolation n7:bacterial%20DNA
n3:kontrolniKodProRIV
[0C07C873DCB5]
n3:mistoKonaniAkce
České Budějovice
n3:mistoVydani
České Budějovice
n3:nazevZdroje
9th Methodological workshop: Present Methods for Investigation of Microbial Community Biodiversity in Soils and Substrates
n3:obor
n12:EE
n3:pocetDomacichTvurcuVysledku
1
n3:pocetTvurcuVysledku
3
n3:rokUplatneniVysledku
n18:2004
n3:tvurceVysledku
Koutný, Marek van Verseveld, H. W. Braster, M.
n3:typAkce
n14:EUR
n3:zahajeniAkce
2004-03-02+01:00
n3:zamer
n20:MSM%20281100002
s:numberOfPages
4
n16:hasPublisher
Ústav půdní biologie AV ČR
n17:organizacniJednotka
28110